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- Creator:
- Burger, Laura L , Wagenmaker, Elizabeth R., Phumsatitpong, Chayarndorn , Olson, David P., and Moenter, Suzanne M.
- Description:
- Polycystic ovary syndrome (PCOS) is the most common form of infertility in women. The causes of PCOS are not yet understood and both genetics and early-life exposure have been considered as candidates. With regard to the latter, circulating androgens are elevated in mid-late gestation in women with PCOS, potentially exposing offspring to elevated androgens in utero; daughters of women with PCOS are at increased risk for developing this disorder. Consistent with these clinical observations, prenatal androgenization (PNA) of several species recapitulates many phenotypes observed in PCOS. There is increasing evidence that symptoms associated with PCOS, including elevated luteinizing hormone (LH) (and presumably gonadotropin-releasing hormone (GnRH)) pulse frequency emerge during the pubertal transition. We utilized translating ribosomal affinity purification coupled with RNA sequencing to examine GnRH neuron mRNAs from prepubertal (3wk) and adult female control and PNA mice. Prominent in GnRH neurons were transcripts associated with protein synthesis and cellular energetics, in particular oxidative phosphorylation. The GnRH neuron transcript profile was affected more by the transition from prepuberty to adulthood than by PNA treatment, however PNA did change the developmental trajectory of GnRH neurons. This included families of transcripts related to both protein synthesis and oxidative phosphorylation, which were more prevalent in adults than in prepubertal mice but were blunted in PNA adults. These findings suggest that prenatal androgen exposure can program alterations in the translatome of GnRH neurons, providing a mechanism independent of changes in the genetic code for altered expression. These are Microsoft Excel Files
- Keyword:
- GnRH Neuron TRAP Seq
- Citation to related publication:
- Unprocessed RNASeq data is available at Gene Expression Omnibus ( https://www.ncbi.nlm.nih.gov/gds) accession GSE155314.
- Discipline:
- Science
-
- Creator:
- Yiwen, Mei
- Description:
- The datasets of this archive are produced for a research project on the development of an advanced hydrologic modeling system for the St. Lawrence river basin. The outputted datasets from model simulations are in Netcdf 4 format. The author recommend using the netCDF Operators (NCO) program for data processing. For visualization and plotting, the author recommend using software like MATLAB, Python or R.
- Keyword:
- Hydrologic modeling, reanalysis product, St. Lawrence river, water balance, WRF-Hydro
- Discipline:
- Science
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- Creator:
- Van, Benjamin J. and Dewaraja, Yuni K.
- Description:
- Interest in quantitative imaging of Y-90 is growing because transarterial radioembolization (RE) with Y-90 loaded microspheres is a promising and minimally invasive treatment that is FDA approved for unresectable primary and metastatic liver tumors. These cancers are a leading cause of cancer mortality and morbidity. Radioembolization is a therapy that irradiates liver tumors with radioactive microspheres administered through a microcatheter placed in the hepatic arterial vasculature. Radioembolization is based on the principle that healthy liver and tumor are mainly vascularized by the portal vein and the hepatic artery respectively. As a result, radioactive microspheres are preferentially located in the lesions after they are administered via the hepatic artery.
- Keyword:
- Y-90, PET, SPECT, CT, Segmentation, Organ, Tumor, Label, Microsphere, Radioembolization, and SIRT
- Citation to related publication:
- Van, B. J., Dewaraja, Y. K., Sangogo, M. L., & Mikell, J. K. (2021). Y-90 SIRT: Evaluation of TCP variation across dosimetric models. EJNMMI Physics, 8(1), 45. https://doi.org/10.1186/s40658-021-00391-6
- Discipline:
- Health Sciences
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- Creator:
- Sergio E. Vidal-Luengo and Mark B. Moldwin
- Description:
- Multi-satellite tracking of solar wind dynamic pressure pulse observations through the Earth's magnetosphere enables us to distinguish local changes with propagation signatures.
- Keyword:
- Heliophysics, Magnetosphere, Dynamic pressure pulse, Magnetosphere, THEMIS, MMS, Cluster, SuperMag, and Heliophysics System Observatory
- Citation to related publication:
- Vidal-Luengo, S. E., & Moldwin, M. B. (2021). Global magnetosphere response to solar wind dynamic pressure pulses during northward IMF using the heliophysics system observatory. Journal of Geophysical Research: Space Physics, 126, e2020JA028587. https://doi.org/10.1029/2020JA028587
- Discipline:
- Science
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- Creator:
- Brasch, Jonathan M, Elipot, Shane, and Arbic, Brian
- Description:
- For Drifters, HYCOM, MITgcm: Spectra and kinetic energy files. Please see readme.txt for a description of all data and code contained here. and - Compare kinetic energies (KE) of high-resolution global ocean models estimated from rotary spectra to KE in surface drifter observations. - Near-inertial KE is closer to drifter observations in models with frequently updated wind forcing - Internal tide KE is closer to drifter observations in models with topographic wave drag
- Keyword:
- oceanography, rotary spectra, kinetic energy, sea surface velocity, and drifters
- Citation to related publication:
- Elipot, S., Lumpkin, R., Perez, R. C., Lilly, J. M., Early, J. J., & Sykulski, A. M. (2016). A global surface drifter data set at hourly resolution. Journal of Geophysical Research: Oceans, 121(5), 2937–2966. https://doi.org/10.1002/2016JC011716
- Discipline:
- Science
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- Creator:
- Mohtat, Peyman, Siegel, Jason B., and Stefanopoulou, Anna G.
- Description:
- The goal here is to study the voltage and expansion response of lithium-ion batteries at different charging rates. Specifically, the goal is to capture the observation of the smoothing of the peaks in dV/dQ and retention of the peaks in d^2 (backslash)delta/dQ^2 at higher C-rates. The retention of the peaks at higher charging rates enables better estimation of the cell capacity. To achieve this goal a reduced order electrochemical and mechanical model with multiple particles with a size distribution is developed. This allows us to capture the smoothing and preservation of the phase transitions in the voltage and expansion measurements at high C-rates, respectively. The model is written in Matlab software.
- Keyword:
- Lithium-ion batteries, Modeling, Multiparticle, Mechanical response, and Electrochemical
- Citation to related publication:
- Mohtat, P., Lee, S., Sulzer, V., Siegel, J. B., & Stefanopoulou, A. G. (2020). Differential Expansion and Voltage Model for Li-ion Batteries at Practical Charging Rates. Journal of The Electrochemical Society, 167(11), 110561. https://doi.org/10.1149/1945-7111/aba5d1
- Discipline:
- Engineering
-
- Creator:
- Vani Archaeological Survey
- Description:
- In 2009, an American-Georgian team of four archaeologists, four archaeology students, and a geophysicist carried out a four-week season of “extensive” survey of known archaeological sites, together with geophysical prospection at selected locations. In a second four-week season in 2010, with the additions of a geologist and an archaeobotanist, we continued our program of documentation of known sites and of exploratory geophysical prospection, and we also carried out limited test excavations at a number of sites. We returned for a shorter (one- to two-week) study seasons in 2011 and 2014, focusing on museum and archival research in Vani, Kutaisi, and Tbilisi., The area covered by our survey extends 15 km both east and west of Vani, and as far as 10 km south of Vani, from the Phasis River plain at approximately 50 m above sea level to the lower slopes of the lesser Caucasus, at approximately 1000 m above sea level. The purpose of the program was to visit all the previously identified archaeological sites in the region, and to integrate existing knowledge into a database of sites and a Geographical Information System. Each entry into this database is recorded as a dataset in this deposit. Entries recorded in 2009 are prefaced with the letter “A,” those recorded in 2010 are prefaced with the letter “B.” A single entry added in 2011 received the preface, “C.” In carrying out the survey, we depended heavily on the unpublished dissertation by Sulkhan Kharabadze, “Vanis Qveq’nis” Arqeologiuri Ruk’a (dzv.ts. VIII – akh.ts. III ss.) – Archaeological Map of the Territory of Vani (8th Century BC – 3rd Century AD) (Ph.D. dissertation: Georgian Technical University 2008). A map showing the locations of all the sites recorded by the survey is attached to this dataset., Our procedure for each site visit was as follows: we drove to the nearest village and searched out a local guide who could take us to the place we wished to see. We drove as far as we could to each site, then got out and walked, using GPS-equipped field computers (Trimble Geo-XM) to make a continuous record of our path. We recorded the lay of the land and any artifacts we saw en route (pottery sherds, traces of burnt daub, lithics and stone objects, architectural features in situ). We designated as points of interest any significant archaeological remains (concentrations of pottery, in situ features, notable stray finds, etc.), and every place we could identify where earlier discoveries had been made or archaeological excavations carried out. For every point of interest, we recorded the latitude, longitude, and elevation; took a series of digital photographs; and made a grab bag collection of pottery and other finds if possible. Where appropriate, we took basic measurements of architectural features (e.g., of Mediaeval towers). We also kept records of local place names, the names of our local guides, and any miscellaneous information they gave us. , Certain sites were selected for further investigation. These included Saqanchia A001, where we carried our geophysical survey and limited excavation; Shuamta, Melashvilebisgora A033, where we also carried out geophysical survey and limited excavation; Kveda Bzvani A047, where we carried out controlled collection of surface finds; and Zeda Bzvani, Meskhebisgora, A053, where we also carried out controlled collection of surface finds., The datasets recorded in this deposit include basic descriptions of each site, citations to previous publications, and links to relevant maps, photographs, and drawings. Where they exist, maps for individual datasets are labeled according to the name and number of the site, e.g., DapnariA002Map.jpg. The labels for photographs taken during the field season record their numbers in the sequence of photographs taken that season, e.g., Vani09.0047.jpg. A complete list of all photographs recorded in this way is available for download. Photographs and drawings of artifacts from individual sites made after the season are labeled with the names of the sites followed by the numbers assigned to the objects, with photographs saved as jpeg files, and drawings saved as tiff files; thus KvedaBzvani11-14.jpg is a photograph of objects 11-14 from the site of KvedaBzvani, while KvedaBzvani11-14.tif is a set of drawings of the same objects. Finally, drawings of sites where excavations were carried out are labeled with the name of the site, the number of the trench (if applicable), and the type of drawing, so that Shuamta2010.1Plan is a plan of Trench 2010.1 at Shuamta., and In addition, the collections in this deposit group datasets together according to important characteristics such as period (Bronze Age, Iron Age, etc.) or type (settlement, fortification, burial, and so on).
- Citation to related publication:
- Kharabadze, S.(2008). “Vanis Qveq’nis” Arqeologiuri Ruk’a (dzv.ts. VIII – akh.ts. III ss.) – Archaeological Map of the Territory of Vani (8th Century BC – 3rd Century AD). (Ph.D. dissertation).Georgian Technical University.
- Discipline:
- Social Sciences and Humanities
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- Creator:
- Chatterjee, Tanmay, Knappik, Achim, Sandford, Erin, Tewari, Muneesh, Choi, Sung Won, Strong, William B., Thrush, Evan P., Oh, Kenneth J., Liu, Ning, Walter, Nils G., and Johnson-Buck, Alexander
- Description:
- The sensitive measurement of specific protein biomarkers is important for medical diagnostics and research. However, existing methods for quantifying proteins use antibody probes that cannot distinguish between specific and nonspecific binding, limiting their sensitivity and specificity. This work establishes a method for distinguishing between specific binding to the target protein and nonspecific binding to assay surfaces using single-molecule kinetic measurements with dynamically binding probes. This is significant because it permits extremely sensitive protein measurements without requiring a high-affinity detection antibody or any washing steps, enabling streamlined and sensitive quantification of proteins even when no pair of high-quality, tightly binding antibodies is available.
- Keyword:
- biomarker detection, single molecule fluorescence, kinetic fingerprinting, total internal reflection microscopy, and super resolution microscopy
- Citation to related publication:
- Chatterjee, T., et al. Direct kinetic fingerprinting and digital counting of single protein molecules. Proc Natl Acad Sci USA, In Press.
- Discipline:
- Science
-
- Creator:
- Sean Sharp
- Description:
- Coastal wetlands intercept significant amounts of nitrogen (N) from watersheds, especially when surrounding land cover is dominated by agriculture and urban development. Through plant uptake, soil immobilization, and denitrification wetlands can remove excess N from flow through water sources and mitigate eutrophication of connected aquatic ecosystems. Excess N can also change plant community composition in wetlands, including communities threatened by invasive species. Understanding how variable hydrology and N loading impact wetland N removal and community composition can help attain desired management outcomes, including optimizing N removal and/or preventing invasion by non-natives. By using a dynamic, process-based ecosystem simulation model, we are able to simulate various levels of hydrology and N loading that would otherwise be difficult to manipulate. We investigate the effects of hydroperiod, hydrologic residence time, N loading, and the NH4+:NO3- ratio on both N removal and the invasion success of two non-native species (Typha x glauca or Phragmites australis) in temperate freshwater coastal wetlands using Mondrian, a process-based, wetland ecosystem simulation model. We found that when residence time increased, annual N removal increased up to 10-fold while longer hydroperiods also increased N removal, but only when residence time was >10 days and N loading was >30 g N m-2 y-1. N removal efficiency also increased with increasing residence time and hydroperiod, but was less affected by N loading. However, longer hydrologic residence time increased vulnerability of wetlands to invasion by both invasive plants at low to medium N loading rates where native communities are typically more resistant to invasion. This suggests a potential tradeoff between ecosystem services related to nitrogen removal and wetland invasibility. These results help elucidate complex interactions of community composition, N loading and hydrology on N removal, helping managers to prioritize N removal when N loading is high or controlling plant invasion in more vulnerable wetlands.
- Keyword:
- Mondrian, Simulation model, Coastal wetlands, Invasive species, and Ecosystem modeling
- Citation to related publication:
- Currie, W. S., Goldberg, D. E., Martina, J., Wildova, R., Farrer, E., & Elgersma, K. J. (2014). Emergence of nutrient-cycling feedbacks related to plant size and invasion success in a wetland community–ecosystem model. Ecological Modelling, 282, 69–82. https://doi.org/10.1016/j.ecolmodel.2014.01.010
- Discipline:
- Science
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- Creator:
- Light, Charles X, Arbic, Brian K, Martin, Paige E, Brodeau, Laurent, Farrar, J Thomas, Griffies, Stephen M, Kirtman, Ben P, Laurindo, Lucas, Menemenlis, Dimitris, Molod, Andrea, Nelson, Arin D, Nyadjro, Ebenezer, O'Rourke, Amanda K, Shriver, Jay, Siqueira, Leo, Small, R Justin, and Strobach, Udi
- Description:
- The precipitation data itself is the output of the models/datasets that we analyze in our paper. Most of it is in .nc or .nc4 format, although we provide code to extract the data into time series .mat files. We used MATLAB to perform our analysis.
- Keyword:
- precipitation and power spectra
- Citation to related publication:
- Light, C.X., Arbic, B.K., Martin, P.E., Brodeau, L., Farrar, J.T., Griffies, S.M., Kirtman, B.P., Laurindo, L.C., Menemenlis, D., Molod, A., Nelson, A.D., Nyadjro, E., O'Rourke, A.K., Shriver, J.F., Siqueira, L., Small, R.J., Strobach, E. (2022). Effects of grid spacing on high-frequency precipitation variance in coupled high-resolution global ocean-atmosphere models. Climate Dynamics, https://doi.org/10.1007/s00382-022-06257-6
- Discipline:
- Science
-
- Creator:
- Zhang, Yan, Fujian Normal University, Yang, Ping, Fujian Normal University, Tong, Chuan, Fujian Normal University, Zhang, Xinyan, Chinese Academy of Sciences, Changchun, Liu, Xingtu, Chinese Academy of Sciences, Changchun, Zhang, Shaoqing, Chinese Academy of Sciences, Changchun, Meyers, Philip. University of Michigan, and Gao, Chuanyu , Chinese Academy of Sciences, Changchun
- Description:
- A high-resolution study of bulk properties in a peat sequence from the Xinjiang Altai Mountains of northwestern China, has allowed reconstruction of local variations in peat properties and peat C and N accumulation rates (CAR and NAR) during the Holocene. Analyses of peat bulk density, loss on ignition, and concentrations of TOC and TN and their elemental ratios and stable isotopic values suggest that changes in peat-forming vegetation types during different parts of this epoch are the major factors responsible for the variations of peat properties in this sequence.
- Keyword:
- peat properties, stable carbon and nitrogen isotopes, carbon and nitrogen accumulation rates, and Altai Mountains of northwestern China
- Citation to related publication:
- Zhang, Y., Yang, P., Gao, C., Tong, C., Zhang, X., Liu, X., Zhang, S., & Meyers, P. A. (2020). Peat Properties and Holocene Carbon and Nitrogen Accumulation Rates in a Peatland in the Xinjiang Altai Mountains, Northwestern China. Journal of Geophysical Research: Biogeosciences, 125(12), e2019JG005615. https://doi.org/10.1029/2019JG005615
- Discipline:
- Science
-
- Creator:
- Li, Jieming, Zhang, Leyou, Johnson-Buck, Alexander, and Walter, Nils G.
- Description:
- Traces from single-molecule fluorescence microscopy (SMFM) experiments exhibit photophysical artifacts that typically necessitate human expert screening, which is time-consuming and introduces potential for user-dependent expectation bias. Here, we have used deep learning to develop a rapid, automatic SMFM trace selector, termed AutoSiM, that improves the sensitivity and specificity of an assay for a DNA point mutation based on single-molecule recognition through equilibrium Poisson sampling (SiMREPS). The improved performance of AutoSiM is based on accepting both more true positives and fewer false positives than the conventional approach of hidden Markov modeling (HMM) followed by thresholding. As a second application, the selector was used for automated screening of single-molecule Förster resonance energy transfer (smFRET) data to identify high-quality traces for further analysis, and achieves ~90% concordance with manual selection while requiring less processing time. AutoSiM can be adapted readily to novel datasets, requiring only modest Transfer Learning.
- Keyword:
- deep learning, single-molecule fluorescence, total internal reflection microscopy, SiMREPS, smFRET, and Forster resonance energy transfer
- Citation to related publication:
- Li, J., Zhang, L., Johnson-Buck, A., & Walter, N. G. (2020). Automatic classification and segmentation of single-molecule fluorescence time traces with deep learning. Nature Communications, 11(1), 5833. https://doi.org/10.1038/s41467-020-19673-1 and Hayward, S., Lund, P., Kang, Q., Johnson-Buck, A., Tewari, M., Walter, N. (2018). Single-molecule microscopy image data and analysis files for "Ultra-specific and Amplification-free Quantification of Mutant DNA by Single-molecule Kinetic Fingerprinting" [Data set]. University of Michigan - Deep Blue. https://doi.org/10.7302/Z2CZ35DF
- Discipline:
- Science
-
Dataset: Models generated for the bioinformatic analysis of MPER/protein fusions binding to antibody
- Creator:
- Bylund, Tatsiana, Chuang, Gwo-Yu, Kwong, Peter, Lai, Yen-Ting, McIlwain, Benjamin, and Stockbridge, Randy B.
- Description:
- This project evaluated the binding of antibody fragments to membrane proteins fused to a short epitope sequence (“MPER”). This dataset includes atomic coordinates (.pdb files) for bioinformatic models of antibody fragment binding to an MPER epitope – membrane protein fusion.
- Keyword:
- MPER, cryo-EM fiducial, crystallography chaperone, and small membrane protein
- Citation to related publication:
- McIlwain, B. C., Erwin, A. L., Davis, A. R., Ben Koff, B., Chang, L., Bylund, T., Chuang, G.-Y., Kwong, P. D., Ohi, M. D., Lai, Y.-T., & Stockbridge, R. B. (2021). N-terminal Transmembrane-Helix Epitope Tag for X-ray Crystallography and Electron Microscopy of Small Membrane Proteins. Journal of Molecular Biology, 166909. https://doi.org/10.1016/j.jmb.2021.166909
- Discipline:
- Science
-
- Creator:
- Attari, Ali
- Description:
- Please refer to the "README.txt" for more details., MATLAB R2018a (Mathworks, Natick, MA, USA) was used to process this data., and Excel (Microsoft Office) was used to store survey data on the comfort of both systems and also to provide absolute and relative intraobserver variablities for the DM device.
- Keyword:
- Digital Manometry
- Citation to related publication:
- Comparison of anorectal function measured using wearable digital manometry and a high resolution manometry system Attari A, Chey WD, Baker JR, Ashton-Miller JA (2020) Comparison of anorectal function measured using wearable digital manometry and a high resolution manometry system. PLOS ONE 15(9): e0228761. https://doi.org/10.1371/journal.pone.0228761
- Discipline:
- Engineering, Science, and Health Sciences
-
- Creator:
- Brandt, Daniel, A., Bussy-Virat, Charles, D., and Ridley, Aaron, J.
- Description:
- The Multifaceted Optimization Algorithm (MOA) is a tool for generating corrected empirical model thermospheric densities during geomagnetic storms. It consists of a suite of Python functions that operate around the Spacecraft Orbit Characterization Kit (SpOCK), an orbital propagator developed by Charles D. Bussy-Virat, PhD, Joel Getchius, and Aaron J. Ridley, PhD at the University of Michigan, and it estimates new densities for the NRLMSISE-00 atmospheric model. MOA generates new model densities by estimating modifications to inputs to the NLRMSISE-00 model that minimize the orbit error between modeled spacecraft in SpOCK, and their actual altitudes as described in publicly-available Two-Line Element Sets (TLEs), made available online via Space-track.org. MOA consists of three sub-process: (1) The Area Optimization Algorithm (AROPT), (2) the F10.7 Optimization Algorithm (FOPT), and (3) the Ap Optimization Algorithm (APOPT). AROPT computes the contribution to the drag of the modeled spacecraft due to their varying projected area. FOPT estimates modifications to the 10.7 cm solar radio flux in NRLMSISE-00, and APOPT estimates modifications to the Earth's magnetic activity in NRLMSISE-00. MOA finds these modifications across many spacecraft, and the medians of those modifications are then applied in NLRMSISE-00 along the orbit of another satellite to generate new densities for verification. In this instance, modifications are applied along the orbits of the Swarm spacecraft and compared to Swarm GPS-derived densities.
- Keyword:
- Orbit, Satellite, Two-line Element Set, Thermosphere, and Drag
- Citation to related publication:
- Brandt, D. A., Bussy-Virat, C. D., & Ridley, A. J. (2020). A Simple Method for Correcting Empirical Model Densities During Geomagnetic Storms Using Satellite Orbit Data. Space Weather, 18(12), e2020SW002565. https://doi.org/10.1029/2020SW002565
- Discipline:
- Engineering
-
- Creator:
- Arbic, Brian K. and Schindelegger, Michael
- Description:
- These netcdf and Matlab files contain the information needed to reproduce Figures 1, 4, 8, 17, 18, 9-16 (minus the proxy values and Monte Carlo results), and the "24 hour" results of Figures 2 and 3.
- Keyword:
- Lunar orbit and tides
- Citation to related publication:
- Daher, H., Arbic, B. K., Williams, J. G., Ansong, J. K., Boggs, D. H., Müller, M., et al. (2021). Long-term Earth-Moon evolution with high-level orbit and ocean tide models. Journal of Geophysical Research: Planets, 126, e2021JE006875. https://doi.org/10.1029/2021JE006875
- Discipline:
- Science
-
- Creator:
- Nunley, Hayden, Nagashima, Mikiko, Martin, Kamirah, Lorenzo Gonzalez, Alcides, Suzuki, Sachihiro C., Norton, Declan A., Wong, Rachel O. L., Raymond, Pamela A., and Lubensky, David K.
- Description:
- This dataset is composed of eight flat-mounted (dissected and fixed) retinae from juvenile and adult zebrafish. Rows of UV cones have been traced in each retina; additionally, we have identified locations of Y-junctions (row insertions). Also included is MATLAB code for calculating which Y-junctions belong to grain boundaries. Please see the readme file for a description of included codes and image files.
- Keyword:
- zebrafish cone mosaic, topological defects, tissue patterning, and grain boundaries
- Citation to related publication:
- Nunley, H., Nagashima, M., Martin, K., Gonzalez, A. L., Suzuki, S. C., Norton, D. A., Wong, R. O. L., Raymond, P. A., & Lubensky, D. K. (2020). Defect patterns on the curved surface of fish retinae suggest a mechanism of cone mosaic formation. PLOS Computational Biology, 16(12), e1008437. https://doi.org/10.1371/journal.pcbi.1008437 and Hayden Nunley, Mikiko Nagashima, Kamirah Martin, Alcides Lorenzo Gonzalez, Sachihiro C. Suzuki, Declan Norton, Rachel O. L. Wong, Pamela A. Raymond, David K. Lubensky. Defect patterns on the curved surface of fish retinae suggest mechanism of cone mosaic formation. bioRxiv 806679; doi: https://doi.org/10.1101/806679
- Discipline:
- Science
-
- Creator:
- Nunley, Hayden, Nagashima, Mikiko, Martin, Kamirah, Lorenzo Gonzalez, Alcides, Suzuki, Sachihiro C., Norton, Declan A., Wong, Rachel O. L., Raymond, Pamela A., and Lubensky, David K.
- Description:
- This dataset contains images of dissected and fixed retinae in which cones of specific subtypes are labeled either by transgenic expression of a fluorescent reporter or by antibody staining (Figures 1 and 2 and 6A and Supplementary Figure 7A). This dataset also contains images of dissected and fixed retinae in ZO1 is immunostained (Figure 6C-E and Supplementary Figure 7B). Please see the readme file for which files correspond to which figures.
- Keyword:
- zebrafish cone mosaic, topological defects, and tissue patterning
- Citation to related publication:
- Nunley, H., Nagashima, M., Martin, K., Gonzalez, A. L., Suzuki, S. C., Norton, D. A., Wong, R. O. L., Raymond, P. A., & Lubensky, D. K. (2020). Defect patterns on the curved surface of fish retinae suggest a mechanism of cone mosaic formation. PLOS Computational Biology, 16(12), e1008437. https://doi.org/10.1371/journal.pcbi.1008437 and Hayden Nunley, Mikiko Nagashima, Kamirah Martin, Alcides Lorenzo Gonzalez, Sachihiro C. Suzuki, Declan Norton, Rachel O. L. Wong, Pamela A. Raymond, David K. Lubensky. Defect patterns on the curved surface of fish retinae suggest mechanism of cone mosaic formation. bioRxiv 806679; doi: https://doi.org/10.1101/806679
- Discipline:
- Science
-
- Creator:
- Nunley, Hayden, Nagashima, Mikiko, Martin, Kamirah, Lorenzo Gonzalez, Alcides, Suzuki, Sachihiro C., Norton, Declan A., Wong, Rachel O. L., Raymond, Pamela A., and Lubensky, David K.
- Description:
- This dataset contains images of UV cone nuclei (labelled by transgenic expression of a photoconvertible fluorescent protein) near the retinal margin in live fish. The most important images in the dataset are the following: 1. Images (at 4X magnification) of UV cones immediately after photoconversion of a patch near the retinal margin 2. Images (at 4X magnification) of UV cones 2-4 days after photoconversion of a patch near the retinal margin Also, included is code for calculating triangulations (which connect UV cone nuclei which are nearest neighbors). This code allows us to check for motion of UV cones relative to each other between the time of photoconversion and subsequent imaging.
- Keyword:
- zebrafish cone mosaic, topological defects, tissue patterning, grain boundaries, photoconversion, and defect motion
- Citation to related publication:
- Nunley, H., Nagashima, M., Martin, K., Gonzalez, A. L., Suzuki, S. C., Norton, D. A., Wong, R. O. L., Raymond, P. A., & Lubensky, D. K. (2020). Defect patterns on the curved surface of fish retinae suggest a mechanism of cone mosaic formation. PLOS Computational Biology, 16(12), e1008437. https://doi.org/10.1371/journal.pcbi.1008437 and Hayden Nunley, Mikiko Nagashima, Kamirah Martin, Alcides Lorenzo Gonzalez, Sachihiro C. Suzuki, Declan Norton, Rachel O. L. Wong, Pamela A. Raymond, David K. Lubensky. Defect patterns on the curved surface of fish retinae suggest mechanism of cone mosaic formation. bioRxiv 806679; doi: https://doi.org/10.1101/806679
- Discipline:
- Science
-
- Creator:
- Nunley, Hayden, Nagashima, Mikiko, Martin, Kamirah, Lorenzo Gonzalez, Alcides, Suzuki, Sachihiro C., Norton, Declan A., Wong, Rachel O. L., Raymond, Pamela A., and Lubensky, David K.
- Description:
- This dataset contains images of UV cone nuclei near the retinal margin in live fish. These UV cones express a transgenic fluorescent reporter (that is nuclear-localized and photoconvertible). The most important images in this dataset are: Zoomed-out (1X magnification) images immediately after photoconversion Zoomed-out (1X magnification) images two to four days after photoconversion In the images immediately after photoconversion, we check if the row orientation rotates by more than a certain amount (10 degrees, 12 degrees, 14 degrees, etc.) at the retinal margin. If so, we call the region coinciding with this domain rotation an existing grain boundary. We, then, check where new Y-junctions are incorporated (by the time of later imaging) to see if they are preferentially incorporated near existing grain boundaries.
- Keyword:
- zebrafish cone mosaic, topological defects, tissue patterning, grain boundaries, and photoconversion
- Citation to related publication:
- Nunley, H., Nagashima, M., Martin, K., Gonzalez, A. L., Suzuki, S. C., Norton, D. A., Wong, R. O. L., Raymond, P. A., & Lubensky, D. K. (2020). Defect patterns on the curved surface of fish retinae suggest a mechanism of cone mosaic formation. PLOS Computational Biology, 16(12), e1008437. https://doi.org/10.1371/journal.pcbi.1008437 and Hayden Nunley, Mikiko Nagashima, Kamirah Martin, Alcides Lorenzo Gonzalez, Sachihiro C. Suzuki, Declan Norton, Rachel O. L. Wong, Pamela A. Raymond, David K. Lubensky. Defect patterns on the curved surface of fish retinae suggest mechanism of cone mosaic formation. bioRxiv 806679; doi: https://doi.org/10.1101/806679
- Discipline:
- Science