http://deepblue.lib.umich.edu/data/concern/data_sets/3b5919491
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Study sites: Information on the location and environmental conditions of the three study sites can be found in: https://doi.org/10.1111/ele.13659
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Trait measurement:
This dataset contains measurements of eleven wood traits of 10 liana and 10 tree seedling species from tropical dry forests. Between two and six seedlings (i.e., 20-70 cm in height, and less than 1 cm in diameter) per species were collected in three mature dry forest in Colombia.
Wood density was calculated, for each stem segment, as fresh volume over dry mass. Fresh volume was measured by the water displacement method, and dry mass was determined after oven-drying the samples to a constant mass at 103º C Cross and tangential anatomical sections (10-15 µm thick) were cut from each stem segment using a rotary microtome with disposable blades (Leica RM2255; Leica Microsystems, Wetzlar, Germany). Anatomical sections were stained in AstraBlue (for 10 min) and Safranine O (for 10 min). Then, they were gradually dehydrated in ethanol series for 1 min each (50%, 75% and 96%), dipped in solvent and mounted on slides with Eukitt mounting medium (Electron Microscopy, Hatfield, PA, USA). Anatomical slides were photographed using a camera (Axiocam 305 color, Zeiss, Germany) mounted on a light microscope (Axio Scope A1, Zeiss). From each anatomical slide, one 100x tangential photograph was taken, while one 10x and one-to-three 40x (depending on stem diameter) photographs were taken in the cross section. To take cross-sectional photographs, we selected in each anatomical slide a representative area covering, in general, most of the radial variation (i.e., from pith to bark) in wood structure, and avoiding visible injuries or tension wood.
In these anatomical photographs, we measured eight wood anatomical traits related to transport, storage and mechanical support (Table 2). To measure the fractions (i.e., % of stem cross-sectional area) of vessels, fibers and parenchyma cells, we first manually colored each cell type in the 10x cross-section photographs using a drawing pad (Wacon CTL-472; China) and Photoshop CS4 (Adobe Systems Incorporated, USA). Then, we calculated the fractions automatically using the batch function in the ImageJ 1.52 software ( http://rsb.info.nih.gov/ij/). When the distinction between fibers and axial parenchyma cells in the cross-sectional photographs was problematic, due to fiber dimorphism and/or thick-walled parenchyma, we checked tangential images to verify the categorization of these cell types. Yet, for two of the species (Phyllanthus botryanthus and “Huevomorrocoy”), we were unable to confidently distinguish axial parenchyma from fibers, thus we excluded these species from the analyses related on fiber and axial parenchyma fractions.
To measure fiber wall thickness (Fwt), each 40x cross-sectional photograph was divided in four equal sections, and 20 fibers per section were randomly selected and measured, for a total of 80 fibers in each photograph. Intervessel pit diameter aperture (Dpm) was measured, in the 100x tangential photographs, as the horizontal pit membrane diameter at its widest point, in 20 pits per vessel and three vessels per photograph. Mean hydraulically weighted vessel diameter (Dh) was measured, in one 10x cross-sectional photograph per individual, as: Dh= (∑ D4/n)1/4, where D is the average of the major and minor axis for each vessel cross-section, and n is the total number of vessels measured . We measured on average 142 vessels per photograph (range 15-455). Vessel diameter variance was calculated using the following formula VarDh = (∑ (Di-D)²)/n-1. Where Di represents the diameter of each vessel, D is the arithmetic mean of all xylem vessel diameters, and n is the total number of xylem vessels measured. Fwt, Dpm and Dh were measured in ImageJ.
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| Description |
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This dataset is part of a research project that aims to study how liana and tree seedlings differ in terms of wood anatomy and demography in three tropical forests in Colombia. These forests are located in the municipalities of Cotove (Antioquia), Colorados (Bolivar) and Tyrona (Magdalena).
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- Other Funding Agency
- National Science Foundation (NSF)
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| Citations to related material |
- González-M., R., Posada, J.M., Carmona, C.P., Garzón, F., Salinas, V., Idárraga-Piedrahita, Á., Pizano, C., Avella, A., López-Camacho, R., Norden, N., Nieto, J., Medina, S.P., Rodríguez-M., G.M., Franke-Ante, R., Torres, A.M., Jurado, R., Cuadros, H., Castaño-Naranjo, A., García, H. and Salgado-Negret, B. (2021), Diverging functional strategies but high sensitivity to an extreme drought in tropical dry forests. Ecology Letters, 24: 451-463. https://doi.org/10.1111/ele.13659
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- https://doi.org/10.7302/c4f7-bn13
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