Date: 10 December, 2024


Dataset Title: Data for Development and prenatal exposure to androgens alter potassium currents in gonadotropin-releasing hormone neurons from female mice


Dataset Creators: Suzanne Moenter, Jennifer Jaime, Anthony DeFazio


Dataset Contact: Suzanne Moenter, smoenter@umich.edu


Funding: National Institutes of Health (NIH)


Description: Deposit contains Deposit contains all data (voltage-clamp recording .dat files from PatchMaster, IgorPro, Prism and Excel files), as well as Adobe Illustrator files of figures, for the associated paper "Development and prenatal exposure to androgens alter potassium currents in gonadotropin-releasing hormone neurons from female mice. "Jaime J, DeFazio RA, Moenter SM. J Neuroendocrinol. 2024 Mar;36(3):e13373. doi: 10.1111/jne.13373.


Methodology: Voltage-clamp recordings of potassium currents from GnRH neurons in brain slices from 3wk old and adult female mice. Mice are either control or prenatally androgenized.


Date Coverage: 20212-11 to 2024-01


Instrument and/or Software specifications: Patchmaster, Igor and Prism softwares used.


Files contained here:
Data within the zipped file are organized into folders

Adult Recordings
- PatchMaster data from adults (as .dat)

CoefVariation
- Analysis of coefficient of variation in Prism and SPSS (as .pzfx, .sav, .spv)

EstrousCycleAnalysis
- Estrous cycle monitoring by mouse - ChiSq_JJCycles.docx

Final PRISM/R Files
- Data analysis in Prism and R (as .prism, .Rhistory)

Igor Pro Data Analysis Files
- Data analysis done in IgorPro (as .pxp)

Juvenile Recordings
- PatchMaster data from juveniles (as .dat)

Single Excel file K+ Current_data.xlsx - consolidation of data in spread sheet

Single Word file K+CurrentAnalysis_Igor_JJ_a.docx - explanation of Igor K Current Analysis Code

Single PowerPoint file KCurrents_ExpDecayFit.pptx - slides showing exponential fit methods

PatchMasterProtocols
- Screen dumps of patchmaster protocols (as .png)

Pilot Recordings
- PatchMaster data from recordings done to work out protocol voltages and duration (as .dat)


Related publication(s):
Development and prenatal exposure to androgens alter potassium currents in gonadotropin-releasing hormone neurons from female mice. Jaime J, DeFazio RA, Moenter SM. J Neuroendocrinol. 2024 Mar;36(3):e13373. doi: 10.1111/jne.13373.


Use and Access: 
This data set is made available under a Creative Commons Attribution-Noncommercial license (CC BY-NC 4.0).


To Cite Data: 
Moenter, S., Jaime, J., DeFazio, A. Data for Development and prenatal exposure to androgens alter potassium currents in gonadotropin-releasing hormone neurons from female mice [Data set], University of Michigan - Deep Blue Data. https://doi.org/10.7302/ptef-4e87