Dataset for analyzing spatial distribution of Y-junctions in flat-mounted retinae The flat mounted retinae included are the following: R4 has 285 (hand-identified) Y-Junctions; R4 is fish #8 (of flat-mounted, row-traced retinae) L1 has 275 (hand-identified) Y-Junctions; L1 is fish #4 (of flat-mounted, row-traced retinae) 7R has 166 (hand-identified) Y-Junctions; 7R is fish #2 (of flat-mounted, row-traced retinae) 5L has 155 (hand-identified) Y-Junctions; 5L is fish #1 (of flat-mounted, row-traced retinae) L11 has 184 (hand-identified) Y-Junctions; L11 is fish #6 (of flat-mounted, row-traced retinae) 9R has 221 (hand-identified) Y-Junctions; 9R is fish #3 (of flat-mounted, row-traced retinae) L2 has 249 (hand-identified) Y-Junctions; L2 is fish #5 (of flat-mounted, row-traced retinae) R3 has 182 (hand-identified) Y-Junctions; R3 is fish #7 (of flat-mounted, row-traced retinae) Pixel size for all of the above images is 0.2227074 µm X 0.2227074 µm To analyze the retina, I recommend: 1. Copying “…_onlylines copy.tif” into a folder called “just_lines” 2. Copying “….tif” (for example, 7R.tif and L2.tif) into a folder called “just_dots” 3. Copying “_full_image.tif” into a folder called “full_images” 4. Copying “….m” files into a folder called “code” The “just_lines” folder contains images with lines along traced rows (no other signal) The “just_dots” folder contains images with dots at every Y-Junction (no other signal) The “full_images” folder contains images with UV cone signal and ZO (zonula occludens) staining. They have distinct layers, so these images are easily editable within photoshop. find_all_defects_in_grain_boundaries_edited.m is the main function for counting which Y-Junctions are in grain boundaries. Please make sure that the “just_lines” and “just_dots” are within the MATLAB path (so that the relevant images may be analyzed). To Cite this Work: Nunley, H., Nagashima, M., Martin, K., Lorenzo Gonzalez, A., Suzuki, S., Norton, D., Wong, R., Raymond, P., Lubensky, D. Dataset for analyzing spatial distribution of Y-junctions in flat-mounted retinae [Data set]. University of Michigan - Deep Blue. https://doi.org/10.7302/jgb4-6h13