This archive provides the data which was analyzed in our manuscript "A Comparison of Lossless Compression Methods in Microscopy Data Storage Applications" which has been submitted for publication.

All images can be opened using Fiji, the open source scientific imaging application [https://imagej.net/software/fiji/].

Authors: LA Walker (ORCID https://orcid.org/0000-0002-5378-3315), Y Li (ORCID https://orcid.org/0000-0002-8647-384X), M McGlothlin (ORCID https://orcid.org/0000-0003-4638-8082), D Cai. (ORCID https://orcid.org/0000-0003-4471-2061)

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Files included in this archive
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1. `bead.tif`
- This is a 3D image of 100nm Invitrogen TetraSpeck fluorescent microspheres imaged in a blue channel using a custom microscope

2. `fly.tif`
- This is a 3D image of a fly Bitbow brain collected as described in Li, et al. Front. Neural Circuits 2021.

3. `neurite.tif`
- This is a 3D image of DiD-labeled mouse V1 tissue, collected using a custom microscope.

4. `noise16.tif`
- This is a collection of frames collected from a sCMOS camera which has been covered to collect an image with only sensor noise.

5. `brainbow.tif`
- This is a mouse Brainbow image originally published and described in Roossien, et al. Bioinformatics 2019.