Date: 12 April, 2023

Dataset Title: 3D Printed Molding of High Aspect Ratio Cell-Impregnated Hydrogel Constructs 

Dataset Creators: O. Habbal, M. Orabi, P. Mohanty, C. Pannier

Dataset Contact: Osama Habbal ohabbal@umich.edu

Funding: N/A


Key Points:
- We design a high aspect ratio biomimetic structure, and construct 3D mold geometry using a desktop fused filament fabrication 3D printer using soluble polyvinyl alcohol (PVA) filament.
- The printed mold is injected with a solution of alginate and Mesenchymal stem cells.
- The cells had a 64% survivability after day 7 in the 3D construct.  


Research Overview:
This research introduces a novel method to produce biomimetic shapes using low cost soluble 3D printed molds. Mesenchymal stem cells in alginate matrix cell viability was studied. The alginate
stem cell structure is made in a construct that is 21 mm wide, 6 mm high, with an arbor diameter of 1 mm (see Combined_Test_Channels.stl). The cells showed 64% survivability at 7 days in the 3D 
constructs.  


Methodology:
- 3D Printed Mold Constructs:
  The mold for the 3D construct was designed in Fusion 360, sliced using CURA V5.0.0 and 
  printed using a Prusa i3 MK3+ using polyvinyl alcohol filament and 0.25 mm airbrush 
  nozzle. The printed molds are sanitized at 120 C and low vacuum of ~27 Torr.
  Fusion360 is a parametric computer aided design (CAD) information about the software 
  can be found on their official website:  autodesk.com/products/fusion-360
  CURA, is an open source 3D printing slicer software that is used to generate .gcode files 
  that most open source 3D printers use to 3D print, more information about CURA can be 
  found on their official website: ultimaker.com/software/ultimaker-cura

- Cell Impregnated Hydrogel 3D Constructs:
  Alginic acid sodium salt 5.0% (w/v) mixed with Mesenchymal stem cells solution was 
  injected into the mold and crosslinked by dipping the filled mold into a calcium chloride solution with sonication, 
  the soluble mold is dissolved and the alginate/cell construct is extracted and incubated at 37 C in DMEM media and 5% CO2 atmosphere.

- Cell Viability Experiments:
  The cell Viability study was conducted using a fluorescent based live/dead assay kit. 


Instrument and/or Software specifications: CURA V5.0.0


Files contained here:
- (Cell_Viability_Experiment_Data.xlsx) Contains the raw experimental data for the cell viability and cell proliferation experiments. 
- (1mm_Combined_Channels.step) Contains the 3D CAD geometry of the 3D construct in .step parametric file format.
- (Combined_Test_Channels.stl) Contains the same geometry in (1mm_Combined_Channels.step) just in .stl mesh file format.
- (Combined_Test_Channels_1mm_Traces_0.25mm_Offset_Funnel_Input_Scaled.stl) Contains the geometry of the mold in .stl mesh file format that was used in the experiments. (NOTE: This the mold geometry 
    for "Combined_Test_Channels.stl")
- (PI3MK3M_Combined_Test_Channels_1mm_Traces_0.25mm_Offset_Funnel_Input.gcode) Contains the .Gcode file used for printing of the mold (NOTE: This file is sliced using CURA V5.0, 
	  it is intended for a PRUSA MK3+ with a 0.25 mm airbrush nozzle and PVA filament. You will need to slow down the feed rate to 50% to be at the correct speed for printing)

Related publication(s):
O Habbal, M Orabi, P Mohanty and C Pannier (2023). 3D Printed Molding of High Aspect Ratio Cell-Impregnated Hydrogel Constructs. Forthcoming.


Use and Access: 
This data set is made available under a Attribution 4.0 International (CC BY 4.0).