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LC/MS/MS analysis of the endogenous dimethyltryptamine hallucinogens, their precursors, and major metabolites in rat pineal gland microdialysate
dc.contributor.authorBarker, Steven A.en_US
dc.contributor.authorBorjigin, Jimoen_US
dc.contributor.authorLomnicka, Izabelaen_US
dc.contributor.authorStrassman, Ricken_US
dc.date.accessioned2013-12-04T18:56:47Z
dc.date.available2015-01-05T13:54:42Zen_US
dc.date.issued2013-12en_US
dc.identifier.citationBarker, Steven A.; Borjigin, Jimo; Lomnicka, Izabela; Strassman, Rick (2013). "LC/MS/MS analysis of the endogenous dimethyltryptamine hallucinogens, their precursors, and major metabolites in rat pineal gland microdialysate." Biomedical Chromatography 27(12): 1690-1700.en_US
dc.identifier.issn0269-3879en_US
dc.identifier.issn1099-0801en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/101767
dc.description.abstractWe report a qualitative liquid chromatography–tandem mass spectrometry (LC/MS/MS) method for the simultaneous analysis of the three known N , N ‐dimethyltryptamine endogenous hallucinogens, their precursors and metabolites, as well as melatonin and its metabolic precursors. The method was characterized using artificial cerebrospinal fluid (aCSF) as the matrix and was subsequently applied to the analysis of rat brain pineal gland‐aCSF microdialysate. The method describes the simultaneous analysis of 23 chemically diverse compounds plus a deuterated internal standard by direct injection, requiring no dilution or extraction of the samples. The results demonstrate that this is a simple, sensitive, specific and direct approach to the qualitative analysis of these compounds in this matrix. The protocol also employs stringent MS confirmatory criteria for the detection and confirmation of the compounds examined, including exact mass measurements. The excellent limits of detection and broad scope make it a valuable research tool for examining the endogenous hallucinogen pathways in the central nervous system. We report here, for the first time, the presence of N , N ‐dimethyltryptamine in pineal gland microdialysate obtained from the rat. Copyright © 2013 John Wiley & Sons, Ltd.en_US
dc.publisherAlan R. Lissen_US
dc.publisherWiley Periodicals, Inc.en_US
dc.subject.otherLC/MS/MSen_US
dc.subject.otherN , N ‐Dimethyltryptaminesen_US
dc.subject.otherMicrodialysisen_US
dc.subject.otherRat Brainen_US
dc.subject.otherPineal Glanden_US
dc.titleLC/MS/MS analysis of the endogenous dimethyltryptamine hallucinogens, their precursors, and major metabolites in rat pineal gland microdialysateen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelPublic Healthen_US
dc.subject.hlbsecondlevelChemistryen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbsecondlevelChemical Engineeringen_US
dc.subject.hlbtoplevelEngineeringen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/101767/1/bmc2981.pdf
dc.identifier.doi10.1002/bmc.2981en_US
dc.identifier.sourceBiomedical Chromatographyen_US
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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