Localized micro‐scale disruption of cells using laser‐generated focused ultrasound

Abstract

We utilize laser‐generated focused ultrasound (LGFU) to create targeted mechanical disturbance on a few cells. The LGFU is transmitted through an optoacoustic lens that converts laser pulses into focused ultrasound. The tight focusing (<100 µm) and high peak pressure of the LGFU produces cavitational disturbances at a localized spot with micro‐jetting and secondary shock‐waves arising from micro‐bubble collapse. We demonstrate that LGFU can be used as a non‐contact, non‐ionizing, high‐precision tool to selectively detach a single cell from its culture substrate. Furthermore, we explore the possibility of biomolecule delivery in a small population of cells targeted by LGFU at pressure amplitudes below and above the cavitation threshold. We experimentally confirm that cavitational disruption is required for delivery of propidium iodide, a membrane‐impermeable nucleic acid‐binding dye, into cells. (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)