Purification, cloning and characterization of the pets factor: A nuclear protein that binds to the inducible TG-rich element of the human immunodeficiency virus type 2 enhancer.
dc.contributor.author | Fu, Glenn Kwei | en_US |
dc.contributor.advisor | Maasab, Hunein F. | en_US |
dc.contributor.advisor | Markovitz, David M. | en_US |
dc.date.accessioned | 2014-02-24T16:24:42Z | |
dc.date.available | 2014-02-24T16:24:42Z | |
dc.date.issued | 1995 | en_US |
dc.identifier.other | (UMI)AAI9624614 | en_US |
dc.identifier.uri | http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqm&rft_dat=xri:pqdiss:9624614 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/104944 | |
dc.description.abstract | The pets site (TTGGTCAGGG) of the human immunodeficiency virus type 2 (HIV-2) enhancer mediates induction in activated T cells. Biochemical characterization and purification demonstrate that the dominant protein binding to the pets site is a 43 kd nuclear protein. Using an enriched Southwestern screening technique the 43 kd HIV-2 pets factor was identified as DEK. DEK has previously been shown to be an autoantigen in certain cases of juvenile onset rheumatoid arthritis and to be joined with the C-terminal portion of the nucleoporin protein CAN (nup214) in a fusion protein specific to certain types of acute myelogenous leukemia (AML). This is the first report of a mechanism of action for DEK, which bears little resemblance to other known proteins and contains no previously described DNA-binding motifs. DEK-CAN is an example of the newly described class of fusion oncoproteins, found in AML, which fuse a nuclear DNA-binding protein with a nucleoporin. DEK is further demonstrated to bind to the pets site in the enhancer of troponin-T (TnT), a cellular gene, as well as HIV-2, and is therefore likely to be the first of a new class of DNA-binding/transcription factors. | en_US |
dc.format.extent | 98 p. | en_US |
dc.subject | Biology, Molecular | en_US |
dc.title | Purification, cloning and characterization of the pets factor: A nuclear protein that binds to the inducible TG-rich element of the human immunodeficiency virus type 2 enhancer. | en_US |
dc.type | Thesis | en_US |
dc.description.thesisdegreename | PhD | en_US |
dc.description.thesisdegreediscipline | Epidemiologic Science | en_US |
dc.description.thesisdegreegrantor | University of Michigan, Horace H. Rackham School of Graduate Studies | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/104944/1/9624614.pdf | |
dc.description.filedescription | Description of 9624614.pdf : Restricted to UM users only. | en_US |
dc.owningcollname | Dissertations and Theses (Ph.D. and Master's) |
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