Isolation and Characterization of Precise Dye/Dendrimer Ratios
dc.contributor.author | Dougherty, Casey A. | en_US |
dc.contributor.author | Furgal, Joseph C. | en_US |
dc.contributor.author | Van dongen, Mallory A. | en_US |
dc.contributor.author | Goodson, Theodore | en_US |
dc.contributor.author | Banaszak holl, Mark M. | en_US |
dc.contributor.author | Manono, Janet | en_US |
dc.contributor.author | DiMaggio, Stassi | en_US |
dc.date.accessioned | 2014-05-23T15:59:54Z | |
dc.date.available | 2015-06-01T15:48:46Z | en_US |
dc.date.issued | 2014-04-14 | en_US |
dc.identifier.citation | Dougherty, Casey A.; Furgal, Joseph C.; Van dongen, Mallory A. ; Goodson, Theodore; Banaszak holl, Mark M. ; Manono, Janet; DiMaggio, Stassi (2014). "Isolation and Characterization of Precise Dye/Dendrimer Ratios." Chemistry â A European Journal 20(16): 4638-4645. | en_US |
dc.identifier.issn | 0947-6539 | en_US |
dc.identifier.issn | 1521-3765 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/106970 | |
dc.description.abstract | Fluorescent dyes are commonly conjugated to nanomaterials for imaging applications using stochastic synthesis conditions that result in a Poisson distribution of dye/particle ratios and therefore a broad range of photophysical and biodistribution properties. We report the isolation and characterization of generation 5 poly(amidoamine) (G5 PAMAM) dendrimer samples containing 1, 2, 3, and 4 fluorescein (FC) or 6‐carboxytetramethylrhodamine succinimidyl ester (TAMRA) dyes per polymer particle. For the fluorescein case, this was achieved by stochastically functionalizing dendrimer with a cyclooctyne “click” ligand, separation into sample containing precisely defined “click” ligand/particle ratios using reverse‐phase high performance liquid chromatography (RP‐HPLC), followed by reaction with excess azide‐functionalized fluorescein dye. For the TAMRA samples, stochastically functionalized dendrimer was directly separated into precise dye/particle ratios using RP‐HPLC. These materials were characterized using 1 H and 19 F NMR spectroscopy, RP‐HPLC, UV/Vis and fluorescence spectroscopy, lifetime measurements, and MALDI. High definition : Two approaches for the formation of generation 5 PAMAM samples containing precise dye/dendrimer ratios are presented. The first approach, using direct separation based on dye hydrophobicity, generated a set of TAMRA‐containing dendrimers, and the second, using click chemistry, generated a set of fluorescein‐containing dendrimer (see figure). | en_US |
dc.publisher | WILEY‐VCH Verlag | en_US |
dc.subject.other | Dendrimers | en_US |
dc.subject.other | Dyes/Pigments | en_US |
dc.subject.other | Fluorescent Probes | en_US |
dc.subject.other | Nanostructures | en_US |
dc.subject.other | Click Chemistry | en_US |
dc.title | Isolation and Characterization of Precise Dye/Dendrimer Ratios | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Chemistry, University of Michigan, Ann Arbor, Michigan, 48109‐1055 (USA) | en_US |
dc.contributor.affiliationother | Department of Chemistry, Xavier University, New Orleans, LA 70125 (USA) | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/106970/1/chem_201304854_sm_miscellaneous_information.pdf | |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/106970/2/4638_ftp.pdf | |
dc.identifier.doi | 10.1002/chem.201304854 | en_US |
dc.identifier.source | Chemistry – A European Journal | en_US |
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