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Far‐Red Organic Fluorophores Contain a Fluorescent Impurity
dc.contributor.authorStone, Matthew B.en_US
dc.contributor.authorVeatch, Sarah L.en_US
dc.date.accessioned2014-08-06T16:49:36Z
dc.date.availableWITHHELD_13_MONTHSen_US
dc.date.available2014-08-06T16:49:36Z
dc.date.issued2014-08-04en_US
dc.identifier.citationStone, Matthew B.; Veatch, Sarah L. (2014). "Far‐Red Organic Fluorophores Contain a Fluorescent Impurity." ChemPhysChem 15(11): 2240-2246.en_US
dc.identifier.issn1439-4235en_US
dc.identifier.issn1439-7641en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/108014
dc.description.abstractFar‐red organic fluorophores commonly used in traditional and super‐resolution localization microscopy are found to contain a fluorescent impurity with green excitation and near‐red emission. This near‐red fluorescent impurity can interfere with some multicolor stochastic optical reconstruction microscopy/photoactivated localization microscopy measurements in live cells and produce subtle artifacts in chemically fixed cells. We additionally describe alternatives to avoid artifacts in super‐resolution localization microscopy. A near‐red fluorescent impurity is characterized in several commonly used far‐red fluorescent dyes. This impurity can lead to artifacts in live‐cell multicolor super‐resolution measurements, subtle artifacts in chemically fixed cells, and highlights the importance of controls in super‐resolution imaging.en_US
dc.publisherWILEY‐VCH Verlagen_US
dc.subject.otherFluorescenceen_US
dc.subject.otherFluorescent Probesen_US
dc.subject.otherLive Cellsen_US
dc.subject.otherQuantitative Imagingen_US
dc.subject.otherSuper‐Resolution Imagingen_US
dc.titleFar‐Red Organic Fluorophores Contain a Fluorescent Impurityen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelPhysicsen_US
dc.subject.hlbsecondlevelChemistryen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Biophysics, University of Michigan, 930 N University, Ann Arbor MI 48109 (USA)en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/108014/1/2240_ftp.pdf
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/108014/2/cphc_201402002_sm_miscellaneous_information.pdf
dc.identifier.doi10.1002/cphc.201402002en_US
dc.identifier.sourceChemPhysChemen_US
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