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S ‐Aryl‐ l ‐cysteine sulphoxides and related organosulphur compounds alter oral biofilm development and AI ‐2‐based cell–cell communication
dc.contributor.authorKasper, S.H.en_US
dc.contributor.authorSamarian, D.en_US
dc.contributor.authorJadhav, A.P.en_US
dc.contributor.authorRickard, A.H.en_US
dc.contributor.authorMusah, R.A.en_US
dc.contributor.authorCady, N.C.en_US
dc.date.accessioned2014-11-04T16:35:40Z
dc.date.availableWITHHELD_13_MONTHSen_US
dc.date.available2014-11-04T16:35:40Z
dc.date.issued2014-11en_US
dc.identifier.citationKasper, S.H.; Samarian, D.; Jadhav, A.P.; Rickard, A.H.; Musah, R.A.; Cady, N.C. (2014). " S ‐Aryl‐ l ‐cysteine sulphoxides and related organosulphur compounds alter oral biofilm development and AI ‐2‐based cell–cell communication." Journal of Applied Microbiology 117(5): 1472-1486.en_US
dc.identifier.issn1364-5072en_US
dc.identifier.issn1365-2672en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/109321
dc.description.abstractAims To design and synthesize a library of structurally related, small molecules related to homologues of compounds produced by the plant Petiveria alliacea and determine their ability to interfere with AI ‐2 cell–cell communication and biofilm formation by oral bacteria. Many human diseases are associated with persistent bacterial biofilms. Oral biofilms (dental plaque) are problematic as they are often associated with tooth decay, periodontal disease and systemic disorders such as heart disease and diabetes. Methods and Results Using a microplate‐based approach, a bio‐inspired small molecule library was screened for anti‐biofilm activity against the oral species Streptococcus mutans UA 159 , Streptococcus sanguis 10556 and Actinomyces oris MG 1. To complement the static screen, a flow‐based BioFlux microfluidic system screen was also performed under conditions representative of the human oral cavity. Several compounds were found to display biofilm inhibitory activity in all three of the oral bacteria tested. These compounds were also shown to inhibit bioluminescence by Vibrio harveyi and were thus inferred to be quorum sensing ( QS ) inhibitors. Conclusion Due to the structural similarity of these compounds to each other, and to key molecules in AI ‐2 biosynthetic pathways, we propose that these molecules potentially reduce biofilm formation via antagonism of QS or QS ‐related pathways. Significance and Impact of the Study This study highlights the potential for a non‐antimicrobial‐based strategy, focused on AI ‐2 cell–cell signalling, to control the development of dental plaque. Considering that many bacterial species use AI ‐2 cell–cell signalling, as well as the increased concern of the use of antimicrobials in healthcare products, such an anti‐biofilm approach could also be used to control biofilms in environments beyond the human oral cavity.en_US
dc.publisherWiley Periodicals, Inc.en_US
dc.subject.otherBiofilmen_US
dc.subject.otherCell–Cell Signallingen_US
dc.subject.otherDental Plaqueen_US
dc.subject.otherMicrofluidicsen_US
dc.subject.otherOralen_US
dc.titleS ‐Aryl‐ l ‐cysteine sulphoxides and related organosulphur compounds alter oral biofilm development and AI ‐2‐based cell–cell communicationen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelMicrobiology and Immunologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/109321/1/jam12616.pdf
dc.identifier.doi10.1111/jam.12616en_US
dc.identifier.sourceJournal of Applied Microbiologyen_US
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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