Effect of Biodentine and Bioaggregate on odontoblastic differentiation via mitogen‐activated protein kinase pathway in human dental pulp cells
dc.contributor.author | Jung, J.‐y. | en_US |
dc.contributor.author | Woo, S.‐m. | en_US |
dc.contributor.author | Lee, B.‐n. | en_US |
dc.contributor.author | Koh, J.‐t. | en_US |
dc.contributor.author | Nör, J. E. | en_US |
dc.contributor.author | Hwang, Y.‐c. | en_US |
dc.date.accessioned | 2015-01-07T15:24:33Z | |
dc.date.available | 2016-04-01T15:21:07Z | en |
dc.date.issued | 2015-02 | en_US |
dc.identifier.citation | Jung, J.‐y. ; Woo, S.‐m. ; Lee, B.‐n. ; Koh, J.‐t. ; Nör, J. E. ; Hwang, Y.‐c. (2015). "Effect of Biodentine and Bioaggregate on odontoblastic differentiation via mitogenâ activated protein kinase pathway in human dental pulp cells." International Endodontic Journal (2): 177-184. | en_US |
dc.identifier.issn | 0143-2885 | en_US |
dc.identifier.issn | 1365-2591 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/110035 | |
dc.description.abstract | Aim To compare the mineralization inductive capacity of Biodentine and Bioaggregate with Mineral trioxide aggregate ( MTA ) and to investigate possible signaling pathways of mineralization in human dental pulp cells ( HDPC s). Methodology Viability of HDPC s in response to Biodentine, Bioaggregate, and MTA was measured using 3‐[4,5‐dimethylthiazol‐2‐yl]‐2,5 diphenyltetrazolium bromide. To investigate their potential to induce odontoblast differentiation, expression of dentine sialophosphoprotein ( DSPP ) and dentine matrix protein1 ( DMP 1) m RNA level was evaluated by RT‐PCR . For the mineralized nodule assay, Alizarin red staining was performed. To determine the role of MAPK signaling in the odontoblastic differentiation of HDPC s, activated MAPK s were investigated by Western blot and the effect of MAPK inhibitor was examined by Alizarin red S staining. The results were statistically analysed using one‐way anova and the Bonferroni test. Results The effects of MTA , Biodentine, and Bioaggregate on cell viability were similar. Biodentine and Bioaggregate enhanced DSPP and DMP 1 m RNA expression compared to the control group, but to the same extent as MTA ( P < 0.05). MTA , Biodentine, and Bioaggregate increased the area of calcified nodules compared to the control ( P < 0.01). MTA , Biodentine, and Bioaggregate increased phosphorylation of extracellular signal‐regulated kinase ( ERK ), p38, and c‐Jun N‐terminal kinase ( JNK ). MAPK inhibitors attenuated mineralized nodule formation, which was increased by MTA , Biodentine, and Bioaggregate, respectively ( P < 0.01). Conclusion Biodentine and Bioaggregate stimulated odontoblastic differentiation and mineralization nodule formation by activating the MAPK pathway as did MTA . This suggests that the new materials could be useful for regenerative endodontic procedures. | en_US |
dc.publisher | Wiley Periodicals, Inc. | en_US |
dc.subject.other | Mineralization | en_US |
dc.subject.other | Odontoblast | en_US |
dc.subject.other | Bioaggregate | en_US |
dc.subject.other | Biodentine | en_US |
dc.subject.other | MAPK | en_US |
dc.title | Effect of Biodentine and Bioaggregate on odontoblastic differentiation via mitogen‐activated protein kinase pathway in human dental pulp cells | en_US |
dc.type | Article | en_US |
dc.rights.robots | IndexNoFollow | en_US |
dc.subject.hlbsecondlevel | Dentistry | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/110035/1/iej12298.pdf | |
dc.identifier.doi | 10.1111/iej.12298 | en_US |
dc.identifier.source | International Endodontic Journal | en_US |
dc.identifier.citedreference | Suzuki S, Haruyama N, Nishimura F, Kulkarni AB ( 2012 ) Dentin sialophosphoprotein and dentin matrix protein‐1: two highly phosphorylated proteins in mineralized tissues. Archive of Oral Biology 57, 1165 – 75. | en_US |
dc.identifier.citedreference | Chang SW, Lee SY, Kum KY, Kim EC ( 2014 ) Effects of ProRoot MTA, Bioaggregate, and Micromega MTA on odontoblast differentiation of human dental pulp cells. Journal of Endodontics 40, 113 – 8. | en_US |
dc.identifier.citedreference | Chung CR, Kim ES, Shin SJ ( 2010 ) Biocompatibility of Bioaggregate cement on human pulp and periodontal ligament (PDL) derived cells. Restorative Dentistry & Endodontics 35, 473 – 8. | en_US |
dc.identifier.citedreference | De‐Deus G, Canabarro A, Alves G, Linhares A, Senne MI, Granjeiro JM ( 2009 ) Optimal cytocompatibility of a bioceramic nanoparticulate cement in primary human mesenchymal cells. Journal of Endodontics 35, 1387 – 90. | en_US |
dc.identifier.citedreference | Hakki SS, Bozkurt SB, Hakki EE, Belli S ( 2009 ) Effects of mineral trioxide aggregate on cell survival, gene expression associated with mineralized tissues, and biomineralization of cementoblasts. Journal of Endodontics 35, 513 – 9. | en_US |
dc.identifier.citedreference | Johnson GL, Lapadat R ( 2002 ) Mitogen‐activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases. Science 298, 1911 – 2. | en_US |
dc.identifier.citedreference | Laurent P, Camps J, About I ( 2012 ) Biodentine(TM) induces TGF‐beta1 release from human pulp cells and early dental pulp mineralization. International Endodontic Journal 45, 439 – 48. | en_US |
dc.identifier.citedreference | Liu H, Lin H, Zhang L et al. ( 2013 ) miR‐145 and miR‐143 regulate odontoblast differentiation through targeting Klf4 and Osx genes in a feedback loop. Journal of Biological Chemistry 288, 9261 – 71. | en_US |
dc.identifier.citedreference | Masuda‐Murakami Y, Kobayashi M, Wang X et al. ( 2010 ) Effects of mineral trioxide aggregate on the differentiation of rat dental pulp cells. Acta Histochemica 112, 452 – 8. | en_US |
dc.identifier.citedreference | Min KS, Yang SH, Kim EC ( 2009 ) The combined effect of mineral trioxide aggregate and enamel matrix derivative on odontoblastic differentiation in human dental pulp cells. Journal of Endodontics 35, 847 – 51. | en_US |
dc.identifier.citedreference | Neary JT ( 1997 ) MAPK cascades in cell growth and death. Physiology 12, 286 – 93. | en_US |
dc.identifier.citedreference | Peng W, Liu W, Zhai W et al. ( 2011 ) Effect of tricalcium silicate on the proliferation and odontogenic differentiation of human dental pulp cells. Journal of Endodontics 37, 1240 – 6. | en_US |
dc.identifier.citedreference | Simon S, Smith AJ, Berdal A, Lumley PJ, Cooper PR ( 2010 ) The MAP kinase pathway is involved in odontoblast stimulation via p38 phosphorylation. Journal of Endodontics 36, 256 – 9. | en_US |
dc.identifier.citedreference | Tani‐Ishii N, Hamada N, Watanabe K, Tujimoto Y, Teranaka T, Umemoto T ( 2007 ) Expression of bone extracellular matrix proteins on osteoblast cells in the presence of mineral trioxide. Journal of Endodontics 33, 836 – 9. | en_US |
dc.identifier.citedreference | Woo SM, Hwang YC, Lim HS et al. ( 2013 ) Effect of nifedipine on the differentiation of human dental pulp cells cultured with mineral trioxide aggregate. Journal of Endodontics 39, 801 – 5. | en_US |
dc.identifier.citedreference | Yasuda Y, Ogawa M, Arakawa T, Kadowaki T, Saito T ( 2008 ) The effect of mineral trioxide aggregate on the mineralization ability of rat dental pulp cells: an in vitro study. Journal of Endodontics 34, 1057 – 60. | en_US |
dc.identifier.citedreference | Yuan Z, Peng B, Jiang H, Bian Z, Yan P ( 2010 ) Effect of Bioaggregate on mineral‐associated gene expression in osteoblast cells. Journal of Endodontics 36, 1145 – 8. | en_US |
dc.identifier.citedreference | Zanini M, Sautier JM, Berdal A, Simon S ( 2012 ) Biodentine induces immortalized murine pulp cell differentiation into odontoblast‐like cells and stimulates biomineralization. Journal of Endodontics 38, 1220 – 6. | en_US |
dc.identifier.citedreference | Zhao X, He W, Song Z, Tong Z, Li S, Ni L ( 2012 ) Mineral trioxide aggregate promotes odontoblastic differentiation via mitogen‐activated protein kinase pathway in human dental pulp stem cells. Molecular Biology Reports 39, 215 – 20. | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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