View Item 

Show simple item record

Effect of an Inhibitor of Glucosylceramide Synthesis on Cultured Human Keratinocytes
dc.contributor.authorTakami, Yoshihiroen_US
dc.contributor.authorAbe, Akiraen_US
dc.contributor.authorMatsuda, Takayoshien_US
dc.contributor.authorShayman, James A.en_US
dc.contributor.authorRadin, Norman S.en_US
dc.contributor.authorWalter, Robert J.en_US
dc.date.accessioned2015-05-04T20:36:08Z
dc.date.available2015-05-04T20:36:08Z
dc.date.issued1998-02en_US
dc.identifier.citationTakami, Yoshihiro; Abe, Akira; Matsuda, Takayoshi; Shayman, James A.; Radin, Norman S.; Walter, Robert J. (1998). "Effect of an Inhibitor of Glucosylceramide Synthesis on Cultured Human Keratinocytes." The Journal of Dermatology 25(2): 73-77.en_US
dc.identifier.issn0385-2407en_US
dc.identifier.issn1346-8138en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/111117
dc.description.abstractGlucosylceramide (GlcCer) is a major glycosphingolipid component of epidermis, which is thought to be related to the barrier function of skin permeability. However, the role of glycosphingolipids in keratinocyte growth and differentiation has not been fully clarified. It has been reported that D‐threo‐1‐phenyl‐2‐decanoylamino‐3‐morpholino‐1‐propanol (PDMP), an inhibitor of GlcCer synthase (EC 2.4.1.80), depletes cells of glycosphingolipids. This inhibitor has been used as a tool for elucidating their functions. In the present study, the effect of PDMP on cultured normal human keratinocytes was investigated. The cells were treated with various concentrations of PDMP. Forty‐eight hours later cell growth, thymidine incorporation, and lipid content were studied. The cell growth and the incorporation of thymidine into cells were inhibited by PDMP in a dose dependent manner. The synthesis of GlcCer was strongly inhibited by PDMP treatment, whereas no significant changes in ceramide level were observed. We concluded that GlcCer in epidermis may play an important role in regulating epidermal growth and suggested that PDMP may be beneficial for treating proliferative skin disorders in the future.en_US
dc.publisherWiley Periodicals, Inc.en_US
dc.subject.otherkeratinocytesen_US
dc.subject.otherglucosylceramideen_US
dc.subject.othercell growthen_US
dc.titleEffect of an Inhibitor of Glucosylceramide Synthesis on Cultured Human Keratinocytesen_US
dc.typeArticleen_US
dc.rights.robotsIndexNoFollowen_US
dc.subject.hlbsecondlevelDermatologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/111117/1/jde02353.pdf
dc.identifier.doi10.1111/j.1346-8138.1998.tb02353.xen_US
dc.identifier.sourceThe Journal of Dermatologyen_US
dc.identifier.citedreferenceBikle DD, Gee E, Pillai S: Regulation of keratinocyte growth, differentiation, and vitamin D metabolism by analog of 1,25‐dehydroxyvitamin D, J Invest Dermatol, 101: 713 – 718, 1993.en_US
dc.identifier.citedreferenceOkazaki T, Bell RM, Hannun YA: Sphimgomyelin turnover induced by vitamin D3 in HL‐60 cells. Role in cell differentiation, J Biol Chem, 264: 19076 – 19080, 1989.en_US
dc.identifier.citedreferenceWakita H, Tokura Y, Yagi H, Nishimura K, Furukawa F, Takigawa M: Keratinocyte differentiation is induced by cell‐permeant ceramides and its proliferation is promoted by sphingosine, Arch Dermatol Res, 286: 350 – 354, 1994.en_US
dc.identifier.citedreferenceHannun YA, Bell RM: Functions of sphingolipids and sphingolipid breakdown products in cellular regulation, Science, 243: 500 – 507, 1989.en_US
dc.identifier.citedreferenceShayman JA: Sphingolipids: Their role in intracellular signaling and renal growth, J Am Soc Nephrol, 7: 171 – 182, 1996.en_US
dc.identifier.citedreferenceInokuchi J, Radin NS: Preparation of the active isomer of 1‐phenyl‐2‐decanoylamino‐3‐morpholino‐1‐propanol, inhibitor of murine glucocerebroside synthase, J Lipid Res, 28: 565 – 571, 1987.en_US
dc.identifier.citedreferenceAbe A, Inokuchi J, Jimbo M, et al: Improved inhibitors of glucosyl; ceramide synthase, J Biochem, 111: 191 – 196, 1992.en_US
dc.identifier.citedreferenceAbe A, Radin NS, Shayman JA, et al: Structural and stereochemical studies of potent inhibitors of glucosyl ceramide synthase and tumor cell growth, J Lipid Res, 36: 611 – 621, 1995.en_US
dc.identifier.citedreferenceUemura K, Sugiyama E, Tamai C, Hara A, Taketomi T, Radin NS: Effect of an inhibitor of glucosylceraminde synthesis on cultured rabbit skin fibroblasts, J Biochem, 108: 525 – 530, 1990.en_US
dc.identifier.citedreferenceInokuchi J, Mason I, Radin NS: Antitumor activity via inhibition of glycosphingolipid biosynthesis, Cancer Lett, 38: 23 – 30, 1987.en_US
dc.identifier.citedreferenceKyogashima M, Inoue M, Seto A, Inokuchi J: Glucosylceramide synthase inhibitor, D‐threo‐1‐phenyl‐2‐decanoylamino‐3‐morpholino‐1‐propanol exhibits a novel decarnogenic activity against Shope carcinoma cells, Cancer Lett, 101: 25 – 30, 1996.en_US
dc.identifier.citedreferenceInokuchi J, Momosaki K, Shimeno H, Nagamatsu A, Radin NS: Effects of D‐threo‐PDMP, an inhibitor of glucosylceramide synthase, on expression of cell surface glycolipid antigen and binding to adhesive proteins by B16 melanoma cells, J Cell Physiol, 141: 573 – 583, 1989.en_US
dc.identifier.citedreferenceInokuchi J, Jimbo M, Momosaki K, Shimeno H, Nagamatsu A, Radin NS: Inhibition of experimental metastasis of murine Lewis lung carcinoma by an inhibitor of glucosylceramide synthase and its possible mechanism of action, Cancer Res, 50: 6731 – 6737, 1990.en_US
dc.identifier.citedreferenceSando GN, Howard EJ, Madison KC: Induction of ceramide glucosyltransferase activity in culture human keratinocytes correlation with culture differentiation, J Biol Chem, 271: 22044 – 22051, 1996.en_US
dc.identifier.citedreferenceBoyce ST, Ham RG: Calcium regulated differentiation of normal human epidermal keratinocytes in chemically defined clonal culture and serum free serial culture, J Invest Dermatol, 81: 33s – 40s, 1983.en_US
dc.identifier.citedreferenceHolleran WM: Lipid modulators of epidermal proliferation and differentiation, Adv Lipid Res, 24: 119 – 139, 1991.en_US
dc.identifier.citedreferenceGray GM, Yardley HJ: Lipid compositions of cells isolated from pig, human, and rat epidermis, J Lipid Res, 16: 434 – 440, 1975.en_US
dc.identifier.citedreferenceLampe MA, Burlingame AL, Whitney J, et al: Human stratum corneum lipids: characterization and regional variations, J Lipid Res, 24: 120 – 130, 1983.en_US
dc.identifier.citedreferenceLampe MA, Williams ML, Elias PM: Human epidermal lipids: characterization and modulations during differentiation, J Lipid Res, 24: 131 – 140, 1983.en_US
dc.identifier.citedreferenceHanaoka S, Takemoto T, Hamanaka Y, et al: Structure determination of glycosphingolipids of cultured human keratinocytes, Biochim Biophys Acta, 1167: 1 – 8, 1993.en_US
dc.identifier.citedreferenceHolleran WM, Feingold KR, Mao‐Qiang M, Gao WN, Lee JM, Elias PM: Regulation of epidermal sphingolipid synthesis by permeability barrier function, J Lipid Res, 32: 1151 – 1158, 1991.en_US
dc.identifier.citedreferenceMarsh NL, Elias PM, Holleran WM: Glucosylceramides stimulate murine epidermal hyperproliferation, J Clinic Invest, 95: 2903 – 2909, 1995.en_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


Files in this item

Name:
jde02353.pdf
Size:
404.0KB
Format:
PDF
View/Open

Show simple item record

Remediation of Harmful Language

The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.

Accessibility

If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.