DEK binding to class II MHC Y-box sequences is gene- and allele-specific
dc.contributor.author | Adams, Barbara S | |
dc.contributor.author | Cha, Hyuk C | |
dc.contributor.author | Cleary, Joanne | |
dc.contributor.author | Haiying, Tan | |
dc.contributor.author | Wang, Hongling | |
dc.contributor.author | Sitwala, Kajal | |
dc.contributor.author | Markovitz, David M | |
dc.date.accessioned | 2015-08-07T17:31:14Z | |
dc.date.available | 2015-08-07T17:31:14Z | |
dc.date.issued | 2003-05-23 | |
dc.identifier.citation | Arthritis Res Ther. 2003 May 23;5(4):R226 | |
dc.identifier.uri | https://hdl.handle.net/2027.42/112485 | en_US |
dc.description.abstract | Abstract Using electrophoretic mobility shift assays, we examined sequence-specific binding of DEK, a potential autoantigen in juvenile rheumatoid arthritis, to conserved Y-box regulatory sequences in class II MHC gene promoters. Nuclear extracts from several cell lines of different phenotypes contained sequence-specific binding activity recognizing DRA, DQA1*0101, and DQA1*0501 Y-box sequences. Participation of both DEK and NF-Y in the DQA1 Y-box binding complex was confirmed by 'supershifting' with anti-DEK and anti-NF-Y antibodies. Recombinant DEK also bound specifically to the DQA1*0101 Y box and to the polymorphic DQA1*0501 Y box, but not to the consensus DRA Y box. Measurement of the apparent dissociation constants demonstrated a two- to fivefold difference in DEK binding to the DQA1 Y-box sequence in comparison with other class II MHC Y-box sequences. Residues that are crucial for DEK binding to the DQA1*0101 Y box were identified by DNase I footprinting. The specific characteristics of DEK binding to these related sequences suggests a potential role for DEK in differential regulation of class II MHC expression, and thus in the pathogenesis of juvenile rheumatoid arthritis and other autoimmune diseases. | |
dc.title | DEK binding to class II MHC Y-box sequences is gene- and allele-specific | |
dc.type | Article | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/112485/1/13075_2002_Article_770.pdf | |
dc.identifier.doi | 10.1186/ar774 | en_US |
dc.language.rfc3066 | en | |
dc.rights.holder | Adams et al., licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL. | |
dc.date.updated | 2015-08-07T17:31:15Z | |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.