Intradiscal injection of simvastatin retards progression of intervertebral disc degeneration induced by stab injury
dc.contributor.author | Zhang, Huina | |
dc.contributor.author | Wang, Lin | |
dc.contributor.author | Park, Jun B | |
dc.contributor.author | Park, Paul | |
dc.contributor.author | Yang, Victor C | |
dc.contributor.author | Hollister, Scott J | |
dc.contributor.author | La Marca, Frank | |
dc.contributor.author | Lin, Chia-Ying | |
dc.date.accessioned | 2015-08-07T17:44:40Z | |
dc.date.available | 2015-08-07T17:44:40Z | |
dc.date.issued | 2009-11-13 | |
dc.identifier.citation | Arthritis Research & Therapy. 2009 Nov 13;11(6):R172 | |
dc.identifier.uri | https://hdl.handle.net/2027.42/112803 | en_US |
dc.description.abstract | Abstract Introduction Earlier work indicates that the cholesterol-lowering drug, simvastatin, is anabolic to chondrogenic expression of rat intervertebral disc (IVD) cells, which suggests a potential role for simvastatin in IVD regeneration. In this study, we expand on our earlier work to test the effectiveness of simvastatin on disc degeneration utilizing a rat tail disc degeneration model. Methods 30 rats that underwent 21 G needle-puncture at rat tail discs were injected with simvastatin-loaded poly(ethylene glycol)-poly(lactic acid-co-glycolic acid)-poly(ethylene glycol) (PEG-PLGA-PEG) gel (5 mg/ml) or vehicle control at 4 weeks after needle injury. All animals were sacrificed 2 weeks after simvastatin injection. Bone morphogenetic protein-2 (BMP-2), aggrecan, collagen type II, and collagen type I messenger ribonucleic acid (mRNA) expression in the rat nucleus pulposus (NP) were measured by real-time polymerase chain reaction (PCR). In vivo magnetic resonance imaging (MRI) was performed to monitor changes in disc degeneration. Rat discs were also assessed by histology using hematoxylin and eosin (H&E) and safranin O staining. In addition, the NP weight, glycosaminoglycan (sGAG) and DNA content were also measured. Results A single dose of simvastatin loaded in thermo-sensitive PEG-PLGA-PEG gel injected into the NP had the trend to increase aggrecan expression and sGAG content, and significantly increased mRNA levels of BMP-2, collagen type II, and the differentiation index (the ratio of collagen type II to collagen type I). The decreased NP weight, T2 intensity, as well as MRI index in the rat tail discs induced by needle puncture were significantly reversed after 2 weeks of simvastatin treatment. In addition, simvastatin treatment also improved histological changes induced by needle puncture. Conclusions A single injection of simvastatin loaded in PEG-PLGA-PEG gel into rat tail discs had the potential to retard or regenerate the degenerative disc. | |
dc.title | Intradiscal injection of simvastatin retards progression of intervertebral disc degeneration induced by stab injury | |
dc.type | Article | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/112803/1/13075_2009_Article_2698.pdf | |
dc.identifier.doi | 10.1186/ar2861 | en_US |
dc.language.rfc3066 | en | |
dc.rights.holder | Zhang et al.. | |
dc.date.updated | 2015-08-07T17:44:40Z | |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.