Interactions between the Translation Machinery and a Translational preQ1 Riboswitch.
dc.contributor.author | Lund, Paul E. | en_US |
dc.date.accessioned | 2016-01-13T18:04:33Z | |
dc.date.available | NO_RESTRICTION | en_US |
dc.date.available | 2016-01-13T18:04:33Z | |
dc.date.issued | 2015 | en_US |
dc.date.submitted | 2015 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/116677 | |
dc.description.abstract | Gene expression is highly regulated with a diversity of regulation at the RNA level. In bacteria, regulation of mRNA translation into protein often occurs through RNA sequence features such as the Shine-Dalgarno (SD) sequence and local structural features. Translational riboswitches in bacteria exemplify such cis-acting regulation. This work look at how structural features of a preQ1 riboswitch effect regulation through interactions with the translation machinery. Broader questions about how individual translational machinery components, such as ribosomal protein S1 and the 30S ribosomal subunit, interact with structured RNAs are also addressed. We sought a more detailed mechanistic view of the interplay between the translational preQ1 riboswitch found in the 5′ UTR of an mRNA from T. tengcongensis, its ligand preQ1, and the SD sequence accessibility. To this end, we developed SiM-KARTS, a generalized strategy to interrogate site-specific structural dynamics of RNA molecules based on probe hybridization kinetics. Intriguingly, we found that the riboswitch expression platform alternates between conformations with differing SD accessibility, which are distinguished by “bursts” of probe binding, the pattern of which is modulated by ligand. This challenges the assumption that riboswitches behave in simple ON/OFF fashion and thus has broader implications for how we think about translational riboswitch regulation. The folding and unfolding of RNA structure influences other cellular processes besides translation. Ribosomal protein S1 performs other roles outside of the context of translation, which are related to its RNA binding or unfolding capacity. We used the well-characterized preQ1 riboswitch as a model pseudoknot to study how S1 interacts with defined, stable tertiary structure. S1 is able to bind and at least partially unfold this pseudoknot in a manner that is limited by RNA structural stability. Lastly, we investigated the influence of S1 on translation of preQ1 riboswitch-containing mRNAs and found that the effects of ligand on translation are not potentiated by the loss of S1. There is, however, a dramatic effect on translational coupling, invoking a role for S1 in polycistronic mRNA translation. These results highlight the need for additional techniques, such as assays at the single molecule level, to monitor early 30S-mRNA interactions during translation. | en_US |
dc.language.iso | en_US | en_US |
dc.subject | preQ1 riboswitch | en_US |
dc.subject | ribosomal protein S1 | en_US |
dc.subject | ribosome translation | en_US |
dc.subject | SiM-KARTS | en_US |
dc.subject | RNA structure | en_US |
dc.title | Interactions between the Translation Machinery and a Translational preQ1 Riboswitch. | en_US |
dc.type | Thesis | en_US |
dc.description.thesisdegreename | PhD | en_US |
dc.description.thesisdegreediscipline | Chemical Biology | en_US |
dc.description.thesisdegreegrantor | University of Michigan, Horace H. Rackham School of Graduate Studies | en_US |
dc.contributor.committeemember | Walter, Nils G. | en_US |
dc.contributor.committeemember | Fierke, Carol A | en_US |
dc.contributor.committeemember | Maddock, Janine R | en_US |
dc.contributor.committeemember | O'Brien, Patrick | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/116677/1/palund_1.pdf | |
dc.owningcollname | Dissertations and Theses (Ph.D. and Master's) |
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