Mechanisms of HIV-1 Nef mediated disruption of MHC-I trafficking.
dc.contributor.author | Kasper, Matthew R. | |
dc.contributor.advisor | Collins, Kathleen L. | |
dc.date.accessioned | 2016-08-30T15:45:15Z | |
dc.date.available | 2016-08-30T15:45:15Z | |
dc.date.issued | 2005 | |
dc.identifier.uri | http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqm&rft_dat=xri:pqdiss:3163838 | |
dc.identifier.uri | https://hdl.handle.net/2027.42/124832 | |
dc.description.abstract | HIVA Nef is a key pathogenic factor necessary for the development of the acquired immunodeficiency syndrome (AIDS). One important function of Nef is to reduce cell surface levels of major histocompatibility complex class I molecules (MHC-I), thereby protecting HIV-infected cells from recognition by cytotoxic T lymphocytes (CTLs). However, the mechanism of MHC-I downmodulation by Nef has not been clearly elucidated. There is evidence that Nef acts early in the secretory pathway to redirect MHC-I from the <italic>trans</italic>-Golgi network to the endolysosomal pathway. However, a competing model suggests that Nef acts much later by accelerating MHC-I turnover at the cell surface. A comparison of Nef's effects on HLA-A2 endocytosis, recycling and transport rate indicated that the most prominent effect of Nef on HLA-A2 in T cells was to inhibit transport to the cell surface. To clarify the mechanism used by Nef in T cells we demonstrated that Nef targets early forms of MHC-1 molecules in the endoplasmic reticulum by preferentially binding hypo-phosphorylated cytoplasmic tails. The Nef-MHC-I complex migrates normally into the Golgi apparatus, but subsequently fails to arrive at the cell surface and become phosphorylated. Cell-type specific differences in the rate of MHC-I transport through the secretory pathway correlate with responsiveness to Nef and coprecipitation of adaptor protein complex-I (AP-1). We propose that the assembly of a Nef-MHC-I-AP-1 complex early in the secretory pathway is important for Nef activity. The ability of Nef to promote this complex causes the diversion of MHC-I transport away from the cell surface and into the endolysosomal pathway, leading to degradation of the MHC-I molecule. | |
dc.format.extent | 151 p. | |
dc.language | English | |
dc.language.iso | EN | |
dc.subject | Disruption | |
dc.subject | Hiv-1 | |
dc.subject | Mechanisms | |
dc.subject | Mediated | |
dc.subject | Mhc Class I | |
dc.subject | Nef | |
dc.subject | Trafficking | |
dc.title | Mechanisms of HIV-1 Nef mediated disruption of MHC-I trafficking. | |
dc.type | Thesis | |
dc.description.thesisdegreename | PhD | en_US |
dc.description.thesisdegreediscipline | Biological Sciences | |
dc.description.thesisdegreediscipline | Microbiology | |
dc.description.thesisdegreegrantor | University of Michigan, Horace H. Rackham School of Graduate Studies | |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/124832/2/3163838.pdf | |
dc.owningcollname | Dissertations and Theses (Ph.D. and Master's) |
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