Fundamental studies of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS) and its applications to the analysis of biological materials.
dc.contributor.author | Zhu, Yongdong | |
dc.contributor.advisor | Lubman, David M. | |
dc.date.accessioned | 2016-08-30T17:53:20Z | |
dc.date.available | 2016-08-30T17:53:20Z | |
dc.date.issued | 1999 | |
dc.identifier.uri | http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqm&rft_dat=xri:pqdiss:9929990 | |
dc.identifier.uri | https://hdl.handle.net/2027.42/131829 | |
dc.description.abstract | This work is focused on the fundamental studies of matrix-assisted laser desorption/ionization mass spectrometry (MALDI/MS) and its application in the detection of biological materials including oligonucleotides, proteins and peptides. The fundamental studies include: the use of active substrates for the detection of DNA in which a modified Nafion film substrate was used to improve the detection of DNA, especially for DNA strands greater than 200 base pairs; the characterization and evaluation of two new matrices (4-hydroxy-3-methoxyphenylpyruvic acid HMPA and indole-3-pyruvic acid EPA) for MALDI/MS in the detection of proteins and peptides; and improvement of mass resolution of MALDI TOF MS by using a simple high voltage transistor switch which can provide high mass resolution up to 1000 for oligonucleotides up to a 60-mer; and by the modification of the oligonucleotide backbone via alkylation of the -S<super>-</super> group on the phosphorothioate oligonucleotides to increase the stability of the oligonucleotide against fragmentation. We also investigated applications of MALDI/MS for the detection of DNA, proteins and peptides which include: development of methodologies for rapid screening of genetic polymorphisms using buccal. cell DNA with detection by MALDI/MS where MALDI/MS can provide more accurate and rapid analysis than the traditional gel electrophoresis method; and development of procedures for off-line coupling between liquid separation methods (CZE and CIEF) with MALDI TOF MS and its applications to the detection of protein and peptide mixtures. | |
dc.format.extent | 164 p. | |
dc.language | English | |
dc.language.iso | EN | |
dc.subject | Analysis | |
dc.subject | Applications | |
dc.subject | Biological | |
dc.subject | Fundamental | |
dc.subject | Ion Trap | |
dc.subject | Maldi | |
dc.subject | Materials | |
dc.subject | Matrix-assisted Laser Desorption/ionization | |
dc.subject | Ms | |
dc.subject | Studies | |
dc.subject | Time-of-flight Mass Spectrometry | |
dc.subject | Tof | |
dc.title | Fundamental studies of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS) and its applications to the analysis of biological materials. | |
dc.type | Thesis | |
dc.description.thesisdegreename | PhD | en_US |
dc.description.thesisdegreediscipline | Analytical chemistry | |
dc.description.thesisdegreediscipline | Pure Sciences | |
dc.description.thesisdegreegrantor | University of Michigan, Horace H. Rackham School of Graduate Studies | |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/131829/2/9929990.pdf | |
dc.owningcollname | Dissertations and Theses (Ph.D. and Master's) |
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