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Protein biomarkers and microbial profiles in periâ implantitis
dc.contributor.authorWang, Hom‐lay
dc.contributor.authorGaraicoa‐pazmino, Carlos
dc.contributor.authorCollins, Amy
dc.contributor.authorOng, Hwen‐sei
dc.contributor.authorChudri, Rini
dc.contributor.authorGiannobile, William V.
dc.date.accessioned2016-10-17T21:17:09Z
dc.date.available2017-11-01T15:31:29Zen
dc.date.issued2016-09
dc.identifier.citationWang, Hom‐lay ; Garaicoa‐pazmino, Carlos ; Collins, Amy; Ong, Hwen‐sei ; Chudri, Rini; Giannobile, William V. (2016). "Protein biomarkers and microbial profiles in periâ implantitis." Clinical Oral Implants Research 27(9): 1129-1136.
dc.identifier.issn0905-7161
dc.identifier.issn1600-0501
dc.identifier.urihttps://hdl.handle.net/2027.42/134099
dc.description.abstractObjectivesThe aim of the present investigation was to determine the profile of periâ implant crevicular fluid (PICF) biomarkers combined with microbial profiles from implants with healthy periâ implant tissues and periâ implantitis to assess realâ time disease activity.Material and methodsSixtyâ eight patients were included in this crossâ sectional study. They were divided into two groups: 34 patients with at least one healthy implant (control) and 34 with at least one periâ implantitis affected implant (test). Total DNA content and qPCR analysis for periodontal bacteria obtained from subgingival plaque samples (Aggregatibacter actinomycetemcomitans, Prevotella intermedia, Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola) and a PICF analysis for ILâ 1β, VEGF, MMPâ 8, TIMPâ 2, and OPG were performed. The individual and combined diagnostic ability of each biomarker for periâ implantitis and target bacterial species were analyzed.ResultsThe mean concentration of ILâ 1β (44.6 vs. 135.8 pg/ml; P < 0.001), TIMPâ 2 (5488.3 vs. 9771.8 pg/ml; P = 0.001), VEGF (59.1 vs. 129.0 pg/ml; P = 0.012), and OPG (66.5 vs. 111.7 pg/ml; P = 0.050) was increased in the periâ implantitis patients. The mean expression of MMPâ 8 (6029.2 vs. 5943.1 pg/ml; P = 0.454) and did not reveal a meaningful difference among groups. Total bacterial DNA of selected microorganisms was associated with a threefold or greater increase in periâ implantitis although no statistical significant difference. The ability to diagnose diseased sites was enhanced by T. denticola combined with ILâ 1β, VEGF, and TIMPâ 2 PICF levels.ConclusionThe present data suggest that the increased levels of the selected PICFâ derived biomarkers of periodontal tissue inflammation, matrix degradation/regulation, and alveolar bone turnover/resorption combined with siteâ specific microbial profiles may be associated with periâ implantitis and could have potential as predictors of periâ implant diseases.
dc.publisherWiley Periodicals, Inc.
dc.subject.othersalivary diagnostics
dc.subject.otherperiâ implant crevicular fluid
dc.subject.otherdental implant microbiology
dc.subject.otherperiâ implant disease
dc.titleProtein biomarkers and microbial profiles in periâ implantitis
dc.typeArticleen_US
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelDentistry
dc.subject.hlbtoplevelHealth Sciences
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/134099/1/clr12708.pdf
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/134099/2/clr12708_am.pdf
dc.identifier.doi10.1111/clr.12708
dc.identifier.sourceClinical Oral Implants Research
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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