Allergens induce enhanced bronchoconstriction and leukotriene production in C5 deficient mice
dc.contributor.author | McKinley, Laura | |
dc.contributor.author | Kim, Jiyoun | |
dc.contributor.author | Bolgos, Gerald L | |
dc.contributor.author | Siddiqui, Javed | |
dc.contributor.author | Remick, Daniel G | |
dc.date.accessioned | 2016-12-05T10:32:09Z | |
dc.date.available | 2016-12-05T10:32:09Z | |
dc.date.issued | 2006-10-17 | |
dc.identifier.citation | Respiratory Research. 2006 Oct 17;7(1):129 | |
dc.identifier.uri | http://dx.doi.org/10.1186/1465-9921-7-129 | |
dc.identifier.uri | https://hdl.handle.net/2027.42/134613 | |
dc.description.abstract | Abstract Background Previous genetic analysis has shown that a deletion in the complement component 5 gene-coding region renders mice more susceptible to allergen-induced airway hyperresponsiveness (AHR) due to reduced IL-12 production. We investigated the role of complement in a murine model of asthma-like pulmonary inflammation. Methods In order to evaluate the role of complement B10 mice either sufficient or deficient in C5 were studied. Both groups of mice immunized and challenged with a house dust extract (HDE) containing high levels of cockroach allergens. Airways hyper-reactivity was determined with whole-body plesthysmography. Bronchoalveolar lavage (BAL) was performed to determine pulmonary cellular recruitment and measure inflammatory mediators. Lung homogenates were assayed for mediators and plasma levels of IgE determined. Pulmonary histology was also evaluated. Results C5-deficient mice showed enhanced AHR to methylcholine challenge, 474% and 91% increase above baseline Penh in C5-deficient and C5-sufficient mice respectively, p < 0.001. IL-12 levels in the lung homogenate (LH) were only slightly reduced and BAL IL-12 was comparable in C5-sufficient and C5-deficient mice. However, C5-deficient mice had significantly higher cysteinyl-leukotriene levels in the BAL fluid, 1913 +/- 246 pg/ml in C5d and 756 +/- 232 pg/ml in C5-sufficient, p = 0.003. Conclusion These data demonstrate that C5-deficient mice show enhanced AHR due to increased production of cysteinyl-leukotrienes. | |
dc.title | Allergens induce enhanced bronchoconstriction and leukotriene production in C5 deficient mice | |
dc.type | Article | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/134613/1/12931_2006_Article_520.pdf | |
dc.language.rfc3066 | en | |
dc.rights.holder | McKinley et al. | |
dc.date.updated | 2016-12-05T10:32:10Z | |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.