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BMP Signaling Mediated by BMPR1A in Osteoclasts Negatively Regulates Osteoblast Mineralization Through Suppression of Cx43
dc.contributor.authorShi, Ce
dc.contributor.authorZhang, Honghao
dc.contributor.authorLouie, Ke’ale
dc.contributor.authorMishina, Yuji
dc.contributor.authorSun, Hongchen
dc.date.accessioned2017-01-10T19:11:09Z
dc.date.available2018-05-04T20:56:57Zen
dc.date.issued2017-03
dc.identifier.citationShi, Ce; Zhang, Honghao; Louie, Ke’ale; Mishina, Yuji; Sun, Hongchen (2017). "BMP Signaling Mediated by BMPR1A in Osteoclasts Negatively Regulates Osteoblast Mineralization Through Suppression of Cx43." Journal of Cellular Biochemistry 118(3): 605-614.
dc.identifier.issn0730-2312
dc.identifier.issn1097-4644
dc.identifier.urihttps://hdl.handle.net/2027.42/135668
dc.description.abstractOsteoblasts and osteoclasts are well orchestrated through different mechanisms of communication during bone remodeling. Previously, we found that osteoclast‐specific disruption of one of the BMP receptors, Bmpr1a, results in increased osteoblastic bone formation in mice. We hypothesized that BMPR1A signaling in osteoclasts regulates production of either membrane bound proteins or secreted molecules that regulated osteoblast differentiation. In our current study, we co‐cultured wild‐type osteoblasts with either control osteoclasts or osteoclasts lacking BMPR1A signaling activity. We found that loss of Bmpr1a in osteoclasts promoted osteoblast mineralization in vitro. Further, we found that the expression of Cx43/Gja1 in the mutant osteoclasts was increased, which encoded for one of the gap junction proteins connexin 43/gap junction alpha 1. Knockdown of Gja1 in the mutant osteoclasts for Bmpr1a reduced osteoblastic mineralization when co‐cultured. Our findings suggest that GJA1 may be one of the downstream targets of BMPR1A signaling in osteoclasts that mediates osteoclast–osteoblast communication during bone remodeling. J. Cell. Biochem. 118: 605–614, 2017. © 2016 Wiley Periodicals, Inc.Disruption of Bmpr1a in osteoclasts promoted osteoblast mineralization when co‐cultured. Up‐regulation of gap junction Cx43/Gja1 in mutant osteoclasts is responsible for the enhanced osteoblast function.
dc.publisherWiley Periodicals, Inc.
dc.subject.otherCONNEXIN 43/GJA1
dc.subject.otherBMPR1A
dc.subject.otherOSTEOCLAST
dc.subject.otherGAP JUNCTION
dc.subject.otherOSTEOBLAST
dc.titleBMP Signaling Mediated by BMPR1A in Osteoclasts Negatively Regulates Osteoblast Mineralization Through Suppression of Cx43
dc.typeArticleen_US
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelGenetics
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biology
dc.subject.hlbtoplevelHealth Sciences
dc.subject.hlbtoplevelScience
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/135668/1/jcb25746_am.pdf
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/135668/2/jcb25746.pdf
dc.identifier.doi10.1002/jcb.25746
dc.identifier.sourceJournal of Cellular Biochemistry
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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