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Absorbable collagen sponges loaded with recombinant bone morphogenetic protein 9 induces greater osteoblast differentiation when compared to bone morphogenetic protein 2
dc.contributor.authorFujioka‐kobayashi, Masako
dc.contributor.authorSchaller, Benoit
dc.contributor.authorSaulacic, Nikola
dc.contributor.authorPippenger, Benjamin E.
dc.contributor.authorZhang, Yufeng
dc.contributor.authorMiron, Richard J.
dc.date.accessioned2017-04-13T20:35:25Z
dc.date.available2018-05-04T20:56:58Zen
dc.date.issued2017-02
dc.identifier.citationFujioka‐kobayashi, Masako ; Schaller, Benoit; Saulacic, Nikola; Pippenger, Benjamin E.; Zhang, Yufeng; Miron, Richard J. (2017). "Absorbable collagen sponges loaded with recombinant bone morphogenetic protein 9 induces greater osteoblast differentiation when compared to bone morphogenetic protein 2." Clinical and Experimental Dental Research 3(1): 32-40.
dc.identifier.issn2057-4347
dc.identifier.issn2057-4347
dc.identifier.urihttps://hdl.handle.net/2027.42/136307
dc.description.abstractThe use of growth factors for the regeneration of soft and hard tissues has been utilized extensively in dental medicine over the past decade. Recently our group found that recombinant human bone morphogenetic protein 9 (rhBMP9) was more osteopromotive than recombinant human bone morphogenetic protein 2 (rhBMP2) when combined with a deprotenized bovine bone mineral bone grafting material. The aim of the present in vitro study was to evaluate the regenerative potential of an absorbable collagen sponge(ACS) specifically designed for extraction socket healing loaded with rhBMP9 when compared to rhBMP2. The adsorption and release kinetics of rhBMP2 and rhBMP9 were first investigated by enzymeâ linked immunosorbent assay quantification. Then, the cellular effects of stromal cell line (ST2) preosteoblasts were investigated utilizing four groups including rhBMP2 and rhBMP9 at both low(10 ng/ml) and high(100 ng/ml) concentrations loaded onto ACS. Cellular attachment(8 hours) and proliferation(1, 3, and 5 days) as well as osteoblast differentiation were investigated by realâ time polymerase chain reaction (PCR) at 3 and 14 days, alkaline phosphatase (ALP) activity at 7 days, and alizarin red staining at 14 days. ACS fully adsorbed both rhBMP2 and rhBMP9 that were slowly released up to 10 days. Although neither rhBMP2 nor rhBMP9 had any effects on cell attachment or proliferation, pronounced effects were observed on osteoblast differentiation. ALP activity was increased sevenâ fold with rhBMP2â high, whereas a marked 10â fold and 20â fold increase was observed with rhBMP9â low and high loaded to ACS, respectively. Furthermore, mRNA levels of collagen1, ALP, bone sialoprotein, and osteocalcin were all significantly higher for rhBMP9 when compared to control or rhBMP2 groups. Alizarin red staining further confirmed that rhBMP9â low and high demonstrated marked increases in mineralization potential when compared to rhBMP2â high. The results demonstrate the marked effect of rhBMP9 on osteoblast differentiation when combined with ACS in comparison to rhBMP2 at doses as much as 10 times lower. Further in vivo studies are necessary to investigate whether the regenerative potential is equally as potent.
dc.publisherWiley Periodicals, Inc.
dc.subject.otherBMP2
dc.subject.otherbone formation
dc.subject.otherBMP9
dc.subject.otherosteoinductive
dc.subject.otherguided bone regeneration
dc.subject.otherGBR
dc.titleAbsorbable collagen sponges loaded with recombinant bone morphogenetic protein 9 induces greater osteoblast differentiation when compared to bone morphogenetic protein 2
dc.typeArticleen_US
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelDentistry
dc.subject.hlbtoplevelHealth Sciences
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/136307/1/cre255.pdf
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/136307/2/cre255_am.pdf
dc.identifier.doi10.1002/cre2.55
dc.identifier.sourceClinical and Experimental Dental Research
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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