Diabetic and idiopathic gastroparesis is associated with loss of CD206‐positive macrophages in the gastric antrum
Grover, M.; Bernard, C. E.; Pasricha, P. J.; Parkman, H. P.; Gibbons, S. J.; Tonascia, J.; Koch, K. L.; McCallum, R. W.; Sarosiek, I.; Hasler, W. L.; Nguyen, L. A. B.; Abell, T. L.; Snape, W. J.; Kendrick, M. L.; Kellogg, T. A.; McKenzie, T. J.; Hamilton, F. A.; Farrugia, G.
2017-06
Citation
Grover, M.; Bernard, C. E.; Pasricha, P. J.; Parkman, H. P.; Gibbons, S. J.; Tonascia, J.; Koch, K. L.; McCallum, R. W.; Sarosiek, I.; Hasler, W. L.; Nguyen, L. A. B.; Abell, T. L.; Snape, W. J.; Kendrick, M. L.; Kellogg, T. A.; McKenzie, T. J.; Hamilton, F. A.; Farrugia, G. (2017). "Diabetic and idiopathic gastroparesis is associated with loss of CD206‐positive macrophages in the gastric antrum." Neurogastroenterology & Motility 29(6): n/a-n/a.
Abstract
BackgroundAnimal studies have increasingly highlighted the role of macrophages in the development of delayed gastric emptying. However, their role in the pathophysiology of human gastroparesis is unclear. Our aim was to determine changes in macrophages and other cell types in the gastric antrum muscularis propria of patients with diabetic and idiopathic gastroparesis.MethodsFull thickness gastric antrum biopsies were obtained from patients enrolled in the Gastroparesis Clinical Research Consortium (11 diabetic, 6 idiopathic) and 5 controls. Immunolabeling and quantitative assessment was done for interstitial cells of Cajal (ICC) (Kit), enteric nerves protein gene product 9.5, neuronal nitric oxide synthase, vasoactive intestinal peptide, substance P, tyrosine hydroxylase), overall immune cells (CD45) and anti‐inflammatory macrophages (CD206). Gastric emptying was assessed using nuclear medicine scintigraphy and symptom severity using the Gastroparesis Cardinal Symptom Index.ResultsBoth diabetic and idiopathic gastroparesis patients showed loss of ICC as compared to controls (Mean [standard error of mean]/hpf: diabetic, 2.28 [0.16]; idiopathic, 2.53 [0.47]; controls, 6.05 [0.62]; P=.004). Overall immune cell population (CD45) was unchanged but there was a loss of anti‐inflammatory macrophages (CD206) in circular muscle (diabetic, 3.87 [0.32]; idiopathic, 4.16 [0.52]; controls, 6.59 [1.09]; P=.04) and myenteric plexus (diabetic, 3.83 [0.27]; idiopathic, 3.59 [0.68]; controls, 7.46 [0.51]; P=.004). There was correlation between the number of ICC and CD206‐positive cells (r=.55, P=.008). Enteric nerves (PGP9.5) were unchanged: diabetic, 33.64 (3.45); idiopathic, 41.26 (6.40); controls, 46.80 (6.04).ConclusionLoss of antral CD206‐positive anti‐inflammatory macrophages is a key feature in human gastroparesis and it is associates with ICC loss.Animal studies have highlighted an important role of macrophages in development of delayed gastric emptying. However, their role in human gastroparesis is unclear. Upon assessment of full thickness gastric antrum biopsies, both diabetic and idiopathic gastroparesis patients showed a loss of CD206‐positive anti‐inflammatory macrophages as compared to controls. This correlated with loss of ICC suggesting a role of innate immune cells in pathophysiology of human gastroparesis.Publisher
Wiley Periodicals, Inc.
ISSN
1350-1925 1365-2982
Other DOIs
Types
Article
Metadata
Show full item recordCollections
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.