Inhibition of CCN6 (WISP3) expression promotes neoplastic progression and enhances the effects of insulin-like growth factor-1 on breast epithelial cells
dc.contributor.author | Zhang, Yanhong | |
dc.contributor.author | Pan, Quintin | |
dc.contributor.author | Zhong, Hui | |
dc.contributor.author | Merajver, Sofia D | |
dc.contributor.author | Kleer, Celina G | |
dc.date.accessioned | 2017-07-02T03:15:47Z | |
dc.date.available | 2017-07-02T03:15:47Z | |
dc.date.issued | 2005-11-08 | |
dc.identifier.citation | Breast Cancer Research. 2005 Nov 08;7(6):R1080 | |
dc.identifier.uri | http://dx.doi.org/10.1186/bcr1351 | |
dc.identifier.uri | https://hdl.handle.net/2027.42/137663 | |
dc.description.abstract | Abstract Introduction CCN6/WISP3 belongs to the CCN (Cyr61, CTGF, Nov) family of genes that contains a conserved insulin-like growth factor (IGF) binding protein motif. CCN6 is a secreted protein lost in 80% of the aggressive inflammatory breast cancers, and can decrease mammary tumor growth in vitro and in vivo. We hypothesized that inhibition of CCN6 might result in the loss of a growth regulatory function that protects mammary epithelial cells from the tumorigenic effects of growth factors, particularly IGF-1. Method We treated human mammary epithelial (HME) cells with a CCN6 hairpin short interfering RNA. Results CCN6-deficient cells showed increased motility and invasiveness, and developed features of epithelial-mesenchymal transition (EMT). Inhibition of CCN6 expression promoted anchorage-independent growth of HME cells and rendered them more responsive to the growth effects of IGF-1, which was coupled with the increased phosphorylation of IGF-1 receptor and insulin receptor substrate-1 (IRS-1). Conclusion Specific stable inhibition of CCN6 expression in HME cells induces EMT, promotes anchorage-independent growth, motility and invasiveness, and sensitizes mammary epithelial cells to the growth effects of IGF-1. | |
dc.title | Inhibition of CCN6 (WISP3) expression promotes neoplastic progression and enhances the effects of insulin-like growth factor-1 on breast epithelial cells | |
dc.type | Article | en_US |
dc.description.bitstreamurl | https://deepblue.lib.umich.edu/bitstream/2027.42/137663/1/13058_2005_Article_1330.pdf | |
dc.language.rfc3066 | en | |
dc.rights.holder | Zhang et al.; licensee BioMed Central Ltd. | |
dc.date.updated | 2017-07-02T03:15:49Z | |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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