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NSUN2‐Mediated m5C Methylation and METTL3/METTL14‐Mediated m6A Methylation Cooperatively Enhance p21 Translation
dc.contributor.authorLi, Qiu
dc.contributor.authorLi, Xiu
dc.contributor.authorTang, Hao
dc.contributor.authorJiang, Bin
dc.contributor.authorDou, Yali
dc.contributor.authorGorospe, Myriam
dc.contributor.authorWang, Wengong
dc.date.accessioned2017-08-01T19:08:52Z
dc.date.available2018-10-02T19:49:01Zen
dc.date.issued2017-09
dc.identifier.citationLi, Qiu; Li, Xiu; Tang, Hao; Jiang, Bin; Dou, Yali; Gorospe, Myriam; Wang, Wengong (2017). "NSUN2‐Mediated m5C Methylation and METTL3/METTL14‐Mediated m6A Methylation Cooperatively Enhance p21 Translation." Journal of Cellular Biochemistry 118(9): 2587-2598.
dc.identifier.issn0730-2312
dc.identifier.issn1097-4644
dc.identifier.urihttps://hdl.handle.net/2027.42/137760
dc.description.abstractN6‐methyladenosine (m6A) and m5C methylation are two major types of RNA methylation, but the impact of joint modifications on the same mRNA is unknown. Here, we show that in p21 3′UTR, NSUN2 catalyzes m5C modification and METTL3/METTL14 catalyzes m6A modification. Interestingly, methylation at m6A by METTL3/METTL14 facilitates the methylation of m5C by NSUN2, and vice versa. NSUN2‐mediated m5C and METTL3/METTL14‐mediated m6A methylation synergistically enhance p21 expression at the translational level, leading to elevated expression of p21 in oxidative stress‐induced cellular senescence. Our findings on p21 mRNA methylation and expression reveal that joint m6A and m5C modification of the same RNA may influence each other, coordinately affecting protein expression patterns. J. Cell. Biochem. 118: 2587–2598, 2017. © 2017 Wiley Periodicals, Inc.In p21 3’UTR,NSUN2 catalyzes m5C modification and METTL3/METTL14 catalyzes m6A modification. Methylation at m6A by METTL3/METTL14 facilitates the methylation of m5C by NSUN2, and vice versa. NSUN2‐mediated m5C and METTL3/METTL14‐mediated m6A methylation synergistically enhance p21 expression at the translational level, leading to elevated expression of p21 in oxidative stress‐induced cellular senescence.
dc.publisherWiley Periodicals, Inc.
dc.subject.otherNSUN2
dc.subject.otherMETTL14
dc.subject.otherp21 mRNA METHYLATION
dc.subject.otherTRANSLATIONAL REGULATION
dc.subject.otherMETTL3
dc.titleNSUN2‐Mediated m5C Methylation and METTL3/METTL14‐Mediated m6A Methylation Cooperatively Enhance p21 Translation
dc.typeArticleen_US
dc.rights.robotsIndexNoFollow
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biology
dc.subject.hlbsecondlevelGenetics
dc.subject.hlbtoplevelHealth Sciences
dc.subject.hlbtoplevelScience
dc.description.peerreviewedPeer Reviewed
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/137760/1/jcb25957.pdf
dc.description.bitstreamurlhttps://deepblue.lib.umich.edu/bitstream/2027.42/137760/2/jcb25957_am.pdf
dc.identifier.doi10.1002/jcb.25957
dc.identifier.sourceJournal of Cellular Biochemistry
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dc.owningcollnameInterdisciplinary and Peer-Reviewed


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