Now showing items 41-50 of 79
Testicular steroid sulfatase: Substrate specificity and inhibition
(Elsevier, 1969-12)
Kinetic studies of the cleavage of dehydroepiandrosterone-sulfate (1) and androstenediol-3-sulfate by a particulate enzyme preparation from a rat testicular microsomal fraction gave Km values of 2.04 x 10-6M for DHA-S ...
in vivo steroid biogenesis and metabolism in the human term placenta 2. in situ placental perfusion with cholesterol-7[alpha]-3H
(Elsevier, 1966-11)
Two human term placentas have been perfused with cholesterol-7[alpha]-3H. The perfusions were carried out at the time of elective repeat cesarean section and tubal ligation in term pregnancies. The major tritiated steroid ...
Automatic continuous monitoring of enzymic activity in column effluent
(Elsevier, 1964-12)
A procedure has been deseribed using the flow system of analysis for continuous monitoring of enzymic activities in column effluents. The system exhibits a high degree of sensitivity. Greater than 90% of the enzyme in the ...
Protein-Carbohydrate interaction : VII. Physical and chemical studies on concanavalin A, the hemagglutinin of the jack bean
(Elsevier, 1968)
Concanavalin A yielded a monodiperse pattern in the analytical ultracentrifuge in the pH range 2-5. The sedimentation coefficient, so20,w in pH 5 acetate buffer containing 0.1 NaCl (total [Gamma]/2 = 0.20) was 4S. At pH ...
A study of the biosynthesis of phenazine-1-carboxylic acid
(Elsevier, 1964)
Washed cell preparations of Pseudomonas aureofaciens synthesize phenazine-1-carboxylic acid in a medium consisting of 0.2 M glycerol and 0.02 M-lysine. Experiments with C14-labeled substrates indicate that all of the carbon ...
Testosterone metabolism in target tissues: Hypothalamic and pituitary tissues of the adult rat and human fetus, and the immature rat epiphysis
(Elsevier, 1969-11)
Rat pituitary, hypothalamic and cerebral cortical minces are demonstrated to effect the conversion of testosterone (T) to 5[alpha]-androstan-17[beta]-o1-3-one (DHT) and [Delta]4-androstene-3,17-dione ([Delta]4A). The ...
A mutant form of ornithine transcarbamylase found in a strain of Neurospora carrying a pyrimidine-proline suppressor gene
(Elsevier, 1962-04)
A mutation, s, of Neurospora, leads to a 98% reduction in ornithine transcarbamylase (OTC) activity without imposing an arginine requirement. It has been found that the enzyme present in the s strain is different from the ...
Enzymic [omega]-oxidation: Stoichiometry of the [omega]-oxidation of fatty acids
(Elsevier, 1969-04)
The stoichiometry of the [omega]-oxidation of fatty acids catalyzed by the inducible enzyme system of Pseudomonas oleovorans was determined. Assay methods are described for the polarographic measurement of oxygen uptake ...
Heterogeneity of tissue dehydrogenases
(Elsevier, 1960-10)
Rat tissue homogenates have been subjected to zone electrophoresis in starch gel. Lactate, malate, isocitrate, glucose 6-phosphate and [alpha]-glycerophosphate dehydrogenase activity was directly visualized by incubating ...