Now showing items 31-40 of 143
Quantitative determination of reducing sugars, oligosaccharides, and glycoproteins with [3H]borohydride
(Elsevier, 1973-09)
A very simple and direct radioactive isotope method has been described for the quantitative determination of reducing sugars. The method can be adapted to give any degree of sensitivity desired. The procedure is of general ...
A shift from phospholipid to triglyceride synthesis when cell division is inhibited by trans-fatty acids
(Elsevier, 1976-10)
The yeast mutant Saccharomyces cerevisiae (KD 46) requires added unsaturated fatty acid for growth. When cell growth was inhibited by the presence of trans-acids there was a marked inhibition of oleate esterification into ...
Affinity binding of the cartilage proteoglycan protein-keratan sulfate core to immobilized hyaluronic acid
(Elsevier, 1978-10-01)
The protein-keratan sulfate core of bovine nasal cartilage proteoglycan was purified by affinity chromatography on a column of immobilized hyaluronic acid. The hyaluronic acid was immobilized by reaction with a hydrazido-alkyl ...
The purification and properties of an aryl [beta]-hexosidase from bovine liver
(Elsevier, 1977-01-30)
An aryl [beta]-hexosidase was purified 800-fold from bovine liver. The purified enzyme hydrolyzed p-nitrophenyl glycosylpyranoside derivatives of [beta]--galactose, [beta]--glucose, [beta]--xylose, [beta]--mannose, and ...
Further studies on the relationship of the stimulatory effects of phenobarbital and 3,4-benzpyrene on hepatic heme synthesis to their effects on hepatic microsomal drug oxidations
(Elsevier, 1970-08)
The administration of either phenobarbital or 3,4-benzpyrene to rats resulted in the rapid and marked induction of [delta]-aminolevulinic acid synthetase (EC 2.3.1.13), the proposed initial and rate-limiting enzyme in the ...
Fractionation of the insoluble brain proteins with acrylamide electrophoresis
(Elsevier, 1970-03)
A method for fractionating the insoluble proteins of the brain with polyacrylamide electrophoresis is described. The procedure consists of dissolving the membrane proteins in a solution of urea, alkali, mercapto-ethanol, ...
Dynamic properties of fatty acid monomolecular films
(Elsevier, 1971-05)
Saturated fatty acids ranging from fifteen to twenty carbons in alkyl chain length were spread as insoluble monomolecular films and compressed continually at constant velocities. As these surface films assume close-packing ...