Now showing items 51-60 of 143
Purification of RNA polymerase from actinomycin producing and nonproducing cells of Streptomyces antibioticus
(Elsevier, 1979-11)
DNA-dependent RNA polymerase has been purified approximately 700-fold from 12-h-old cells of Streptomyces antibioticus and 400-fold from 48-h cells. Both enzymes appear nearly homogeneous as judged by sodium dodecyl ...
Quantitative analysis of pyridine nucleotides in red blood cells: A single-step extraction procedure
(Elsevier, 1976-03)
Quantitative analysis of red cell pyridine nucleotides has been unreliable in the past because of technical problems in extracting them in the presence of hemoglobin. A simple alcoholic extraction procedure for analysis ...
Natural occurrence of S-alkyl glycerol ethers in ehrlich ascites cells
(Elsevier, 1978)
1. 1. S-alkyl glycerol ethers were isolated by thin-layer chromatography from the total lipids of Ehrlich ascites cells following reduction with vitride.2. 2. Gas-liquid chromatography of the isopropylidene derivatives ...
Assay of glycogen phosphorylase by differential spectropolarimetry
(Elsevier, 1971-09)
A differential spectropolarimetric method has been developed for the measurement of glycogen phosphorylase activity. The reaction course measured in this way was first order, in agreement with results obtained by measuring ...
A sensitive fluorescent method for the detection of glycoproteins in polyacrylamide gels
(Elsevier, 1976-05-21)
A sensitive method is described for the detection of glycoproteins after polyacrylamide gel electrophoresis. Dansyl hydrazine is condensed with aldehydic functions generated by periodate oxidation of protein bound carbohydrate. ...
Analysis of data from experiments using double labelling
(Elsevier, 1978-01)
Frequently as a result of experiments in which two isotopes are used one is left with a sequence of samples, the ratio of labeling in each sample, and the problem of analyzing the ratios. Suppose that the experiments are ...
Studies on the initiation of protein synthesis in mouse myeloma tumors
(Elsevier, 1975)
35S- and 3H-labeled short, nascent peptides have been extracted from mouse myeloma ribosomes after incubation of myeloma fragments with [35S]methionine and [3H]amino acids. Edman analysis of these peptides reveals that ...
Contact-shifted NMR of spinach ferredoxin: Additional resonances and partial assignments
(Elsevier, 1972-06)
We have extended the study of the contact-shifted resonances in the nmr spectra of oxidized and reduced spinach ferredoxin to lower magnetic field than reported by others. We have found two resonances not observed previously: ...
An immobilized naphthylamide substrate for proteinases with tryptic-like specifleity
(Elsevier, 1977-12)
An immobilized amino acid naphthylamide substrate for proteinases with tryptic-like specificity was prepared by reacting -arginine [beta]-naphthylamide with an N-hydroxysuccinimide-activated derivative of agarose. Hydrolysis ...
Immobilized glucose oxidase and -amino acid oxidase: A convenient method for the purification of flavin adenine dinucleotide and its analogs
(Elsevier, 1979-05)
The preparation of flavin adenine dinucleotide-affinity columns employing glucose oxidase and -amino acid oxidase covalently linked to Sepharose-4B is described. Both immobilized enzymes have very good long-term stabilities, ...