Now showing items 61-70 of 143
Regulatory effects of steroids on the pituitary response to LH-RH
(Elsevier, 1975-06)
The pituitary response to a continuous i.v. LH-RH administration (15 [mu]g/h/3 h) associated with physiological or pharmacological doses of several steroid hormones such as estradiol (E2), progesterone, 17[alpha]OH-progesterone, ...
O-sulfate esters of hydroxy amino acids in hydrolyzates of proteoglycans
(Elsevier, 1972-12)
In the preparation of hydrolyzates of proteoglycans for analysis of amino acids, ninhydrin-positive materials other than free amino acids can be generated. Some of these are O-sulfate esters of the hydroxy amino acids and ...
A method of measuring protein dry weight in solutions of unknown salt concentration and an application to lipoproteins
(Elsevier, 1973-05)
The dialysis bag dry weight method was developed for the measurement of dry weights of lipoproteins subfractionated on density gradients where total recovery of lipoprotein in the gradient was to be determined. Use of the ...
Effect of metal-complexing agents on the oxygenase activity of sheep vesicular glands
(Elsevier, 1973-12)
A variety of agents that are known to complex copper can reversibly inhibit the oxygenation of unsaturated fatty acids by particulate preparations from sheep vesicular glands. The time-dependent activation of the oxygenase ...
Lipid extraction of tissues with a low-toxicity solvent
(Elsevier, 1978-10-01)
An improved method for extracting the lipids from tissues consists of the use of hexane:isopropanol, followed by a wash of the extract with aqueous sodium sulfate to remove nonlipid contaminants. This method has a number ...
Radioiodination of sulfhydryl-sensitive proteins
(Elsevier, 1975-08)
A new procedure is described for the radioiodination of proteins with sulfhydryl groups essential for their biological activity. Aniline is iodinated with 125I-labeled sodium iodide in the presence of chloramine-T, the ...
Decrease in specific activity of heart and muscle aldolase in old mice
(Elsevier, 1977)
1. 1. Aldolase was purified from mouse heart and muscle cells by column chromatography. The enzyme from 3- and 30-month old mouse tissues was assayed under different temperature and pH conditions in order to determine the ...
Effects of atractyloside on the incorporation of radioactive precursors into DNA in isolated rat liver mitochondria
(Elsevier, 1970-12)
Atractyloside (20 [mu]) has been found to decrease the incorporation of dATP-3H into DNA by 90% in intact mitochondria (at a level of 3 mg of protein) but to have no such effect on sonic extracts of mitochondria nor on the ...
A microscale isolation of hemins from hemeproteins by use of polyacrylamide gel electrophoresis
(Elsevier, 1975)
An analytical and semipreparative procedure has been developed for the isolation of the prosthetic groups of various hemeproteins by use of polyacrylamide gel electrophoresis. Electrophoresis of erythrocyte formylhemeprotein ...
Lead ion activates phosphorylation of electroplax Na+- and K+- dependent adenosine triphosphatase [(NaK)-ATPase] in the absence of sodium ion
(Elsevier, 1976-06)
PbCl2 in micromolar concentrations stimulates phosphorylation of electroplax microsomal protein in the absence of Na+. Other divalent cations showed little or no such effect. The (Mg2+ + Pb2+)- and (Mg2+ + Na+)-dependent ...