Now showing items 1-10 of 38
Development of hypothalamic opioid neurons: A combined immunocytochemical and [3H]thymidine autoradiographic study
(Elsevier, 1985-02)
Using a combined technique of immunocytochemistry and [3H]thymidine autoradiography, we have determined the "birth-date" of opioid peptide containing neurons in three hypothalamic nuclei. These include proopiomelanocortin ...
Enkephalinase: Selective inhibitors and partial characterization
(Elsevier, 1980)
There are at least two types of enzymes in brain, endopeptidases and aminopeptidases, which metabolize enkephalins. Evidence is presented to suggest that enkephalinase, an endopeptidase cleaving at the Gly-Phe bond, is ...
A sensitive coupled HPLC/RIA technique for separation of endorphins: Multiple forms of B-endorphin in rat pituitary intermediate vs. anterior lobe
(Elsevier, 1981-08)
A technique is described which couples High Performance Liquid Chromatography (HPLC) to radioimmuno assays (RIA), thus achieving high sensitivity with good specificity. This method allows the separation and detection of ...
Electroencephalographic assessment of the role of [delta] receptors in opioid peptide - induced seizures
(Elsevier, 1984-12)
DPDPE ([D-Pen2, D-Pen5]-Enkephalin) and DPLPE ([D-Pen2, L-Pen5]-Enkephalin) are conformationally-constrained cyclic analogs of enkephalin with high selectivity for [delta] opioid receptors. Intracerebroventricular (i.c.v.) ...
Strategies for studying opioid peptide regulation at the gene, message and protein levels
(Elsevier, 1984)
Three opioid peptide precursors have been isolated and characterized in endocrine and nervous tissue: pro-opiomelanocortin, pro-enkephalin, and pro-dynorphin. Since each of those opioid peptide systems have been extensively ...
Anticonvulsant effects of mu (DAGO) and delta (DPDPE) enkephalins in rats
(Elsevier, 1988)
The effects of highly selective mu and delta opioid peptide agonists were determined in two rat models of experimentally-induced convulsions, the flurothyl threshold test and the maximal electroshock test. Intracerebroventricular ...
Postnatal development of beta-endorphin immunoreactivity in the medulla oblongata of rat
(Elsevier, 1985-02)
Beta-endorphin (B-END) like immunoreactivity (i.r.) levels were measured by radioimmunoassay in the medulla oblongata of developing rats on postnatal ages P1-P42 at 7 day intervals, and in adult rats. From P1 to P42, B-END ...
Dynorphin reduces voltage-dependent calcium conductance of mouse dorsal root ganglion neurons
(Elsevier, 1984-12)
Dynorphin A (DYN) (1 [mu]M) decreased somatic calcium-dependent action potential (CAP) duration of a portion of dorsal root ganglion (DRG) neurons in a naloxone reversible manner. Responses to DYN differed from responses ...
Role of central epinephrine in regulation of anterior pituitary hormone secretion
(Elsevier, 1982)
Hypothalamic regulation of anterior pituitary hormones is thought to be mediated by the release of stimulatory and/or inhibitory peptides that are, in turn, regulated by catecholaminergic neurons. The recent development ...
The preferential release of beta-endorphin from the anterior pituitary lobe by Corticotropin Releasing Factor (CRF)
(Elsevier, 1986)
Although a number of investigators have shown that release of ACTH is accompanied by the release of Beta-endorphin ([beta]-End) and Beta-lipotropin ([beta]-LPH), the proportion of the latter two peptides released with ...