Now showing items 231-240 of 261
Glycosyltransferase activities of Ehrlich ascites tumor cells: Detection, isolation, and characterization using oligosaccharide-Synsorb beads
(Elsevier, 1987-04)
Detergent extracts of Ehrlich tumor cell membranes exhibit a host of glycosyltransferase activities which have been investigated using oligosaccharides immobilized to Synsorb beads as acceptors. Glycosidase digestions in ...
Genetic variation in the carbonic anhydrase isozymes of macaque monkeys. IV. Degradation by heat and proteolysis of normal and variant carbonic anhydrase isozymes of Macaca nemestrina
(Elsevier, 1984-04)
Studies were undertaken on the heat denaturation and proteolytic degradation by [alpha]-chymotrypsin of the normal red cell carbonic anhydrase isozyme, CA II, and two electrophoretic variants of carbonic anhydrase I, CA ...
Improved high-performance liquid chromatographic assay for the quantification of 5-bromo-2'-deoxyuridine and 5-bromouracil in plasma
(Elsevier, 1988)
A sensitive and specific procedure using high-performance liquid chromatography (HPLC) was developed for the quantification of 5-bromo-2'-deoxyuridine (BUdR) and 5-bromouracil (BU) in plasma. BUdR and BU were first extracted ...
Two-dimensional peptide mapping by reversed-phase column chromatography, applied to the sequence determination of cytochrome c from the wild type and a mutant of the butterfly, pieris brassicae
(Elsevier, 1983-08-26)
Two-dimensional peptide mapping has been very effective in the characterization of protein digests, particularly for the detection of small structural differences between homologous proteins. The classical thin-layer ...
[beta]-Endorphin suppression of acute morphine abstinence in morphine dependent monkeys: Effective given intraventricularly but ineffective given intravenously
(Elsevier, 1985-07)
Six female adult monkeys were made dependent upon morphine sulfate and were implanted with a chronic indwelling needle in the lateral ventricle of the brain for sterile intraventricular injections. Both [beta]-endorphin ...
Prodynorphin peptide immunocytochemistry in rhesus monkey brain
(Elsevier, 1985)
The present study describes the immunocytochemical distribution of peptides derived from the prodynorphin precursor in the brain of the rhesus monkey (Macaca mulatta). Animals were treated with colchicine (intracerebrove ...
Evidence that removal of an endogenous metal that stabilizes the untransformed glucocorticoid receptor in cytosol allows ligand-independent receptor transformation
(Elsevier, 1989)
Cytosol preparations contain an endogenous heat-stable factor which stabilizes the glucocorticoid receptor in its untransformed, non DNA-binding form. Elution of a partially purified preparation of this stabilizing factor ...
Analysis of sperm antigens by sodium dodecyl sulfate gel/protein blot radioimmunobinding method
(Elsevier, 1982-06)
A radioimmunobinding method based on the blotting of renatured proteins from sodium dodecyl sulfate gels on to nitrocellulose filter papers was developed to analyze the sperm antigens that elicit serum anti-sperm antibodies. ...
Determination of the dead time of a stopped-flow fluorometer
(Elsevier, 1989-09)
This investigation was carried out to develop a convenient alternative method for examining the performance and determining the dead time of a stopped-flow fluorometer. We examined the kinetics for the formation of the ...
Crossed immunoelectrophoresis from sodium dodecyl sulfate-polyacrylamide gels after fixation and staining and without nonionic detergent intermediate layers
(Elsevier, 1981-09-01)
A modified method is described for crossed immunoelectrophoresis in which the first-dimension separation has been carried out by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The described method does not ...