Now showing items 241-250 of 261
Pituitary-adrenal mediation of bombesin-induced inhibition of gastrointestinal transit in rats
(Elsevier, 1984-11)
Centrally administered bombesin (0.1-3.5 [mu]g, i.c.v.) inhibits gastrointestinal transit of a charcoal meal in a dose-related manner in rats. The roles of pituitary and adrenal glands in the mediation of this effect were ...
Iron-ethylenediaminetetraacetic acid (EDTA)-catalyzed superoxide dismutation revisited: An explanation of why the dismutase activity of Fe-EDTA cannot be detected in the cytochrome c/xanthine oxidase assay system
(Elsevier, 1982-05)
The recent assertion of [1.] that Fe-EDTA does not catalyze superoxide dismutation is disputed. By directly observing superoxide generated during pulse radiolysis, we have confirmed the results of a previous study ( [2.]) ...
Decreased pulmonary vascular responsiveness in rats raised on an essential fatty acid deficient diet
(Elsevier, 1987-02)
Leukotriene C4 is produced during hypoxic pulmonary vasoconstriction and leukotriene inhibitors preferentially inhibit the hypoxic pressor response in rats. If lipoxygenase products are important in hypoxic vasoconstriction, ...
Potentiometric study of cytochrome c1aa3 from thermus thermophilus
(Elsevier, 1985)
We have examined the redox behavior of the cytochrome c1aa3 complex from Thermus thermophilus. In potentiometric titrations the cytochrome c behaves as an independent center having n = 1 and E = 205 mV (NHE). Under the ...
An assay for the rates of cleavage of specific sites in DNA by restriction endonucleases: Its use to study the cleavage of phage [lambda] DNA by EcoRI and phage P22 DNA containing thymine or 5-bromouracil by HindIII
(Elsevier, 1983-03)
A method to measure the rates of cleavage of specific sites in DNAs by restriction endonucleases is described. Partial digests are prepared by incubating DNAs with limiting amounts of endonuclease. The termini generated ...
Measurement of nucleoside kinases in crude tissue extracts
(Elsevier, 1983-08)
The measurements of deoxyadenosine kinase, adenosine kinase, and deoxycytidine kinase were examined in human placental cytosol to achieve a valid and reliable assay linear with time and protein. Our studies confirm the ...
Radiometric assay for direct quantitation of rat liver cytochrome P-450b using monoclonal antibodies
(Elsevier, 1985-08-15)
A simple and sensitive assay has been developed that is capable of detecting as little as 0.2 ng of the major isozyme of cytochrome P-450 (P-450b) isolated from the livers of phenobarbital-induced rats. This assay employs ...
Cumene hydroperoxide-supported denitrification of 2-nitropropane in uninduced mouse liver microsomes
(Elsevier, 1986)
1. 1. Cumene hydroperoxide supported oxidative denitrification of 2-nitropropane was investigated in uninduced mouse liver microsomes.2. 2. The cytochrome P-450 peroxygenase catalyzed reaction resulted in the production ...