Now showing items 31-40 of 261
Dopamine [beta]-hydroxylation in diabetes and diabetic autonomic neuropathy
(Elsevier, 1981-08)
Dopamine [beta]-hydroxylase (DBH) has been studied in healthy subjects and diabetic patients, some of whom had autonomic neuropathy. DBH was assayed by a recently described in vivo tritium release method (10,11). Dopamine ...
Paired-ion high-performance liquid chromatographic assay for plasma choline
(Elsevier, 1980-03-14)
Choline was isolated from deproteinized plasma by cation-exchange chromatography. Isolated choline was directly converted to the 3,5-dinitrobenzoate derivative and was analyzed by paired-ion high-performance liquid ...
Agarose drop method for loading thin polyacrylamide gels
(Elsevier, 1982-11-01)
The gels (<1 mm) can be loaded conveniently, rapidly, and quantitatively by suspending the sample to be analyzed in a drop of agarose gel and simply placing the solidified drop on top of the stacking gel. With this ...
Oxygen radicals in complement and neutrophil-mediated acute lung injury
(Elsevier, 1985)
The development of experimental acute lung injury following systemic complement activation is closely related to availability of blood neutrophils. Although tissue-destructive neutrophil-derived may play a supportive role ...
Simple, rapid, and highly efficient separation of amino acid phenylthiohydantoins by reversed-phase high-performance liquid chromatography
(Elsevier, 1982-04)
A rapid and efficient separation of amino acid phenylthiohydantoins by high-performance liquid chromatography has been accomplished by step-gradient elution with use of a mobile phase of acetate-buffered aqueous acetonitrile ...
Purification of the pancreatic cholecystokinin receptor
(Elsevier, 1989-03)
We have previously shown that the pancreatic cholecystokinin (CCK) receptor can be solubilized in 1% digitonin. In this study, digitonin-solubilized CCK receptors from rat pancreas were purified using sequential affinity ...
Detection of electrophoretically silent mutations by immobilized pH gradients
(Elsevier, 1986)
The detection of neutral amino acid mutants, by isoelectric focusing in immobilized pH gradients (IPGs), is exemplified by the separation of hemoglobin (Hb) Beirut (126[beta] Val --> Ala) from Hb A in a shallow pH 7.2-7.6 ...
Transfer of small plasmid DNA fragments from polyacrylamide gels onto nitrocellulose paper
(Elsevier, 1983-10-15)
The incorporation into a 7% polyacrylamide gel of a nucleic acid-specific photochemical reagent 4,5',8-trimethylpsoralen at a concentration of 1 mg/dl of acrylamide solution improves both the sensitivity and the efficiency ...
Ornithine carbamyl transferase in Reye's syndrome
(Elsevier, 1984-12)
Serum levels of ornithine carbamyl transferase activities were determined in the acutely ill and convalescent Reye's syndrome patients and in their parents. Acutely ill Reye's syndrome patients had elevated levels of serum ...