Now showing items 11-20 of 21
Chemical extraction of indigo from Indigofera tinctoria while attaining biological integrity
(Kluwer Academic Publishers; Springer Science+Business Media, 1999-08)
Indigofera tinctoria was permeabilized with 20% methanol (v/v) at 25 °C and released 8±2 μg indigo g −1 dry plant material (excluding roots). This is equivalent to 42±11% of the total indican within the cells. The plants ...
Observations consistent with autocrine stimulation of hybridoma cell growth and implications for large-scale antibody production
(Kluwer Academic Publishers; Springer Science+Business Media, 1992-04)
Existence of autocrine growth factors (aGFs) may influence the serum requirement for growth of hybridoma cells and thus significantly influence process economics. For the murine hybridoma cell line S3H5/γ2bA2, critical ...
[alpha]-(2 --> 3)- and [alpha]-(2 --> 6)-Sialyltransferase activities present in three variants of Ehrlich tumor cells: identification of the products derived from N-acetyllactosamine and [beta]--Gal-(1 --> 3)-[alpha] GalNAc-(1 --> O)-Bn
(Elsevier, 1994-11-01)
We compared several sialyltransferase activities related to synthesis of O-linked and N-linked sialylglycoproteins in Ehrlich ascites tumor cells that grow normally in murine ascites, but are not adherent nor grow in tissue ...
Control of gene expression at the level of translation initiation
(Elsevier, 1994-10)
Protein synthesis is controlled at the level of translation initiation. Cells rapidly respond to environmental changes by disassembly of polysomes and recruitment of specific mRNAs from inactive ribonucleoprotein particles ...
Effect of calcium chloride treatment on hybridoma cell viability and growth
(Kluwer Academic Publishers; Springer Science+Business Media, 1992-10)
In order to minimize hybridoma cell damage during calcium alginate entrapment, the effect of calcium chloride treatment on hybridoma cell viability and growth was studied in terms of calcium chloride concentration and ...
A Comparison of Mushroom Tyrosinase Dopaquinone and Dopachrome Assays Using Diode-Array Spectrophotometry: Dopachrome Formation vs Ascorbate-Linked Dopaquinone Reduction
(Elsevier, 1993-02)
Comparisons of two assay methods for the catecholase activity of mushroom tyrosinase (EC 1.14.18.1) are reported. Tyrosinase (or polyphenol oxidase) is a widely distributed copper-containing enzyme which possesses both ...
Simplified method of making alginate-polylysine microcapsules for hybridoma cell culture using RPMI 1640 medium
(Kluwer Academic Publishers; Science & Technology Letters ; Springer Science+Business Media, 1990-09)
The method of making alginate-poly-L-lysine microcapsules for hybridoma cell culture can be simplified by cultivating the cells in RPMI 1640 medium. Phosphate concentration in RPMI 1640 medium is sufficiently high to ...