Now showing items 11-20 of 46
Protamine immobilization and heparin adsorption on the protamine-bound cellulose fiber membrane
(Wiley Subscription Services, Inc., A Wiley Company, 1992-02-20)
Immobilization of protamine to the inner lumen of cellulose hollow fibers has been shown useful in preventing both heparin- and protamine-induced complications during an extracorporeal blood circulation procedure. The ...
Irreversible enzyme-shuttle immunoassay
(Wiley Subscription Services, Inc., A Wiley Company, 1992-03-25)
The concept of a competitive enzyme immunoassay that utilizes simultaneously the bound and the free analyte-enzyme conjugate (heterobifunctional conjugate) for signal generation in response to varying analyte concentrations ...
Degradation kinetics of pentachlorophenol by Phanerochaete chrysosporium
(Wiley Subscription Services, Inc., A Wiley Company, 1990-05)
The extracellular enzymes and cell mass from the pregrown Phanerochaete chrysosporium cultures were used for the degradation of PCP. The use of both extracellular enzymes and cell mass resulted in extensive mineralization ...
1-Monoglyceride production from lipase-catalyzed esterification of glycerol and fatty acid in reverse micelles
(Wiley Subscription Services, Inc., A Wiley Company, 1991-08-20)
Glycerol-fatty acid esterification has been conducted with lipase from R. delemar in water/AOT/isooctane reverse micellar media, with the major product being 1-monoglyceride, a useful food-emulsifier. 1,3-diglyceride was ...
Frequent harvesting from perfused bone marrow cultures results in increased overall cell and progenitor expansion
(Wiley Subscription Services, Inc., A Wiley Company, 1994-08-20)
The establishment of prolific long-term human bone marrow cultures has led to the development of hematopoietic bioreactor systems. A single batch expansion of bone marrow mononuclear cell populations leads to a 10- to ...
Effects of ammonia and lactate on hybridoma growth, metabolism, and antibody production
(Wiley Subscription Services, Inc., A Wiley Company, 1992-02-20)
The influence of ammonia and lactate on cell growth, metabolic, and antibody production rates was investigated for murine hybridoma cell line 163.4G5.3 during batch culture. The specific growth rate was reduced by one-half ...
Microencapsulated human bone marrow cultures: A potential culture system for the clonal outgrowth of hematopoietic progenitor cells
(Wiley Subscription Services, Inc., A Wiley Company, 1994-04-05)
Currently the most successful methods for culturing human hematopoietic cells employ some form of perfused bioreactor system. However, these systems do not permit the clonal outgrowth of single progenitor cells. Therefore, ...
Optimizing seeding and culture methods to engineer smooth muscle tissue on biodegradable polymer matrices
(Wiley Subscription Services, Inc., A Wiley Company, 1998-01-05)
The engineering of functional smooth muscle (SM) tissue is critical if one hopes to successfully replace the large number of tissues containing an SM component with engineered equivalents. This study reports on the effects ...
Loss of antibody productivity is highly reproducible in multiple hybridoma subclones
(Wiley Subscription Services, Inc., A Wiley Company, 1993-06-20)
An immunoglobulin G (IgG 2b ) producing hybridoma cell line (S3H5/Γ2bA2) was cloned and subcloned. Twenty subclones were grown in parallel while being adapted in a stepwise fashion to serum-free medium. Following adaptation ...
Countercurrent gradient chromatography: A continuous focusing technique
(Wiley Subscription Services, Inc., A Wiley Company, 1995-12-05)
The continuous separation of proteins was performed in a countercurrent gradient chromatography (CGC) system. A magnetically stabilized fluidized bed (MSFB) was used to establish true countercurrent contact of a solid resin ...