Now showing items 361-370 of 386
A self-renewal assay for cancer stem cells
(Springer-Verlag, 2005-11)
Cancers of epithelial origin are responsible for the majority of cancer-related deaths in the USA. Unfortunately, although chemotherapy and/or radiation therapy can sometimes shrink tumors, metastatic cancers of epithelial ...
In the fluorescent spotlight: Global and local conformational changes of small catalytic RNAs
(Wiley Subscription Services, Inc., A Wiley Company, 2002)
RNA is a ubiquitous biopolymer that performs a multitude of essential cellular functions involving the maintenance, transfer, and processing of genetic information. RNA is unique in that it can carry both genetic information ...
Analysis of Oxidized DNA Fragments by Gel Electrophoresis
(Wiley Periodicals, Inc., 2001-04)
Polyacrylamide gel electrophoresis is used to define and quantify products of deoxyribose oxidation in DNA, based on the unique electrophoretic mobility of DNA fragments possessing deoxyribose oxidation products on their ...
NMR Determination of Oligonucleotide Structure
(Oxford University PressWiley Periodicals, Inc., 2000-02)
This unit provides an overview of the use of NMR to determine oligonucleotide structure. It covers basic NMR spectral properties, acquisition of interproton distance restraints and torsion angle restraints, structure ...
Solid‐Phase Synthesis of Branched Oligonucleotides
(Wiley Periodicals, Inc.Academic Press, 2002-06)
Branched nucleic acids (bNAs) have been of particular interest since the discovery of RNA forks and lariats as intermediates of nuclear mRNA splicing, as well as multicopy, single‐stranded DNA (msDNA). Such molecules contain ...
Chemical and Enzymatic Methods for Preparing Circular Single‐Stranded DNAs
(CRC PressWiley Periodicals, Inc., 2000-02)
Small circular oligonucleotides can be used for diagnostic, therapeutic, and laboratory purposes. These systems have gained considerable attention in recent years because they form unusually strong and specific complexes ...
3′‐Modified Oligonucleotides and their Conjugates
(Wiley Periodicals, Inc.Pierce Chemical Company, 2000-12)
Solid‐phase synthesis of 3′‐aminoalkyl, 3′‐thioalkyl, and 3′‐polyethyleneglycol are described. The first two are important as specific points of attachment for a large variety of reporter groups; the latter is important ...
Probing Nucleic Acid Structure with Nickel‐ and Cobalt‐Based Reagents
(Kluwer Academic PublishersWiley Periodicals, Inc., 2000-02)
The use of nickel and cobalt reagents is presented for characterizing the solvent exposure of guanine residues in DNA and RNA. These reagents promote guanine oxidation in the presence of a peracid such as monopersulfate, ...
Titelbild: Heterochromia in Designed Metallopeptides: Geometry-Selective Binding of Cd II in a De 14Novo Peptide (Angew. Chem. 35/2007)
(WILEY-VCH Verlag, 2007-09-03)
No Abstract
Empirical entropic contributions in computational docking: Evaluation in APS reductase complexes
(Wiley Subscription Services, Inc., A Wiley Company, 2008-08)
The results from reiterated docking experiments may be used to evaluate an empirical vibrational entropy of binding in ligand–protein complexes. We have tested several methods for evaluating the vibrational contribution ...