Now showing items 21-30 of 313
Base Composition Analysis of Nucleosides Using HPLC
(Applied Biosystems,Wiley Periodicals, Inc., 2000-07)
In this protocol, nuclease digestion of an oligonucleotide is followed by dephosphorylation and HPLC analysis of the monomers on a reversed‐phase C18 column. This method can be used to detect and quantitate a wide variety ...
Synthesis and Erosion Properties of PEG-Containing Polyanhydrides
(WILEY-VCH Verlag, 2007-05-10)
To tailor the erosion rate of polyanhydrides while retaining their surface erosion characteristics, new three-component polyanhydrides of sebacic acid, 1,3-bis( p -carboxyphenoxy)propane and poly(ethylene glycol) were ...
Chemoenzymatic Preparation of Nucleoside Triphosphates
(Wiley Periodicals, Inc., 2004-03)
The design and synthesis of alternative nucleoside triphosphate substrates for DNA and RNA polymerases holds continued promise to create biochemical probes and precursors for synthesis of nucleic acid mimics. The azole ...
Engineering new bone tissue in vitro on highly porous poly(Α-hydroxyl acids)/hydroxyapatite composite scaffolds
(John Wiley & Sons, Inc., 2001-02)
Engineering new bone tissue with cells and a synthetic extracellular matrix (scaffolding) represents a new approach for the regeneration of mineralized tissues compared with the transplantation of bone (autografts or ...
Modification of the 5′ Terminus of Oligonucleotides for Attachment of Reporter and Conjugate Groups
(John Wiley & Sons, 2000-02)
Reporter and conjugate groups can be added directly to the 5′ terminus of oligonucleotides by appropriate modification. Conjugate groups can be used to increase the affinity of complementary strands, induce irreversible ...
Nano-fibrous scaffolding architecture selectively enhances protein adsorption contributing to cell attachment
(Wiley Subscription Services, Inc., A Wiley Company, 2003-11-01)
Tissue engineering aims at resolving problems such as donor shortage and immune rejection faced by transplantation. Scaffolds (artificial extracellular matrices) have critical roles in tissue engineering. Recently, we ...
Rotator Cuff Tendinosis in an Animal Model: Role of Extrinsic and Overuse Factors
(Kluwer Academic Publishers-Plenum Publishers; Biomedical Engineering Society ; Springer Science+Business Media, 2002-09)
The rat shoulder animal model has been used previously to study the role of intrinsic injury (modeled as an acute insult to the tendon), extrinsic injury (modeled as external subacromial impingement), and overuse factors ...
Protection of 2′‐Hydroxy Functions of Ribonucleosides
(Oxford University PressWiley Periodicals, Inc., 2000-02)
The main purpose of this article is to discuss 2′‐protection in the context of effective oligoribonucleotide synthesis. Emphasis is placed on the 2′‐protecting groups of choice in the synthesis of oligo‐and polyribonucleotides, ...
Comparative proteomic study of two closely related ovarian endometrioid adenocarcinoma cell lines using cIEF fractionation and pathway analysis
(WILEY-VCH Verlag, 2009-04)
The proteomic profiles from two distinct ovarian endometrioid tumor-derived cell lines, (MDAH-2774 and TOV-112D) each with different morphological characteristics and genetic mutations, have been studied. Characterization ...