Now showing items 21-30 of 31
A computational approach to inferring cellular protein-binding affinities from quantitative fluorescence resonance energy transfer imaging
(WILEY-VCH Verlag, 2009-12)
Fluorescence resonance energy transfer (FRET) microscopy can measure the spatial distribution of protein interactions inside live cells. Such experiments give rise to complex data sets with many images of single cells, ...
Toward high sequence coverage of proteins in human breast cancer cells using on-line monolith-based HPLC-ESI-TOF MS compared to CE MS
(WILEY-VCH Verlag, 2006-06)
A method is developed toward high sequence coverage of proteins isolated from human breast cancer MCF10 cell lines using a 2-D liquid separations. Monolithic-capillary columns prepared by copolymerizing styrene with ...
CE of poly(amidoamine) succinamic acid dendrimers using a poly(vinyl alcohol)-coated capillary
(WILEY-VCH Verlag, 2008-01)
Various generations (G1–G8) of negatively charged poly(amidoamine) (PAMAM) succinamic acid dendrimers (PAMAM-SAH) were analyzed by CE using a poly(vinyl alcohol)-coated capillary. Due to its excellent stability and osmotic ...
Electrophoretic mobility and molecular distribution studies of poly(amidoamine) dendrimers of defined charges
(WILEY-VCH Verlag, 2006-05)
Generation 5 ethylenediamine (EDA)-cored poly(amidoamine) (PAMAM) dendrimers (E5, E denotes the EDA core and 5 the generation number) with different degrees of acetylation and carboxylation were synthesized and used as a ...
BiomarkerDigger: A versatile disease proteome database and analysis platform for the identification of plasma cancer biomarkers
(WILEY-VCH Verlag, 2009-07)
We have developed a proteome database (DB), BiomarkerDigger ( http://biomarkerdigger.org ) that automates data analysis, searching, and metadata-gathering function. The metadata-gathering function searches proteome DBs for ...
Validation of reverse phase protein array for practical screening of potential biomarkers in serum and plasma: Accurate detection of CA19-9 levels in pancreatic cancer
(WILEY-VCH Verlag, 2008-08)
The current study analyzed reverse phase protein arrays (RPPA) as a means to experimentally validate biomarkers in blood samples. One microliter samples of sera ( n 14= 1471), and plasma ( n 14= 1478) were serially diluted ...
Multiplexed detection and applications for separations on parallel microchips
(WILEY-VCH Verlag, 2008-08)
Much work has been performed since the development of the lab-on-a-chip concept that has brought microfabricated systems to the forefront of bioanalytical research. The success of using these microchips for performing ...
Strategies for plasma proteomic profiling of cancers
(WILEY-VCH Verlag, 2006-10)
Despite a voluminous literature on potential protein biomarkers and a compelling need for diagnostic tests based on biomarkers to detect cancers at much earlier, more treatable stages, progress has been limited. New methods ...
A proteomic analysis of Psychrobacter articus 273-4 adaptation to low temperature and salinity using a 2-D liquid mapping approach
(WILEY-VCH Verlag, 2007-02)
Psychrobacter 273-4 was isolated from a 20 000–40 000–year-old Siberian permafrost core, which is characterized by low temperature, low water activity, and high salinity. To explore how 273-4 survives in the permafrost ...
Automated integration of monolith-based protein separation with on-plate digestion for mass spectrometric analysis of esophageal adenocarcinoma human epithelial samples
(WILEY-VCH Verlag, 2006-09)
A unique approach of automating the integration of monolithic capillary HPLC-based protein separation and on-plate digestion for subsequent MALDI-MS analysis has been developed. All liquid-handling procedures were performed ...