Now showing items 21-30 of 159
Protection of 5′‐Hydroxy Functions of Nucleosides
(IRL PressWiley Periodicals, Inc., 2000-02)
The 5‐hydroxy group is the primary hydroxy group of nucleosides. It is mandatory to protect 5‐hydroxyls in all methods of oligonucleotide synthesis that require nucleoside synthons. This unit discusses a wide variety of ...
Characterization of Tertiary Folding of RNA by Circular Dichroism and Urea
(Wiley Periodicals, Inc., 2001-04)
CD spectroscopy can be used to monitor RNA tertiary folding transitions that may not be observable by absorbance spectroscopy. With the use of computer‐controlled titrators, data can be acquired rapidly, and accurate ...
Biophysical Analysis of Nucleic Acids
(VCH PublishersWiley Periodicals, Inc., 2000-02)
This overview unit provides a thorough overview of biophysical methods used for structure analysis, including X‐ray diffraction, nuclear magnetic resonance, optical spectroscopy, theoretical and computational methods, and ...
References to Commonly Used Techniques
(John Wiley & Sons, Inc, 2000-02)
Many of the protocols in this manual assume a knowledge of basic (chemical and biochemical) techniques. Although some of these are outside the scope of Current Protocols in Nucleic Acid Chemistry (CPNC), several basic ...
Column Chromatography
(John Wiley & Sons,, 2000-12)
Column chromatography is used extensively in nucleic acid chemistry to purify or characterize products from chemical reactions. This appendix focuses on separations using silica gel or alumina. The procedures described ...
A Base‐Labile Protecting Group (Fluorenylmethoxycarbonyl) for the 5′‐Hydroxy Function of Nucleosides
(Wiley Periodicals, Inc.IRL Press, 2000-02)
Many popular synthesis strategies look for appropriate 2′‐O‐protection methods to use in conjunction with 5′‐O‐trityl chemistry. In contrast, this unit describes the use of FMOC as a 5′‐protecting group in conjunction with ...
Preparation of 5′‐Silyl‐2′‐Orthoester Ribonucleosides for Use in Oligoribonucleotide Synthesis
(Wiley Periodicals, Inc., 2004-03)
The recent discovery that small interfering RNAs (siRNAs) induce gene suppression in mammalian cells has sparked tremendous interest in using siRNA‐based assays and high‐throughput screens to study gene function. As a ...
Stepwise Solid‐Phase Synthesis of Nucleopeptides
(Wiley Periodicals, Inc.Academic Press, 2004-03)
Phosphodiester‐linked peptide‐oligonucleotide conjugates (nucleopeptides) are obtained by stepwise solid‐phase procedures. The peptide is first assembled on a suitably derivatized solid matrix and the oligonucleotide is ...