Now showing items 91-100 of 160
Synthesis of Oligonucleotide Conjugates via Aqueous Diels‐Alder Cycloaddition
(Wiley Periodicals, Inc.Cold Spring Harbor Laboratory Press, 2003-09)
The conjugation of maleimide reporter groups to 5′‐diene‐modified oligonucleotides via aqueous Diels‐Alder cycloaddition is described. Detailed basic protocols are provided for the preparation of a diene‐amidite (5′‐diene ...
Synthesis and Polymerase Incorporation of 5′‐Amino‐2′,5′‐Dideoxy‐5′‐N‐Triphosphate Nucleotides
(Wiley Periodicals, Inc., 2004-09)
This unit presents synthetic procedures for the preparation of 5′‐amino‐2′,5′‐dideoxy analogs of adenosine, cytidine, guanosine, and thymidine, as well as corresponding 5′‐N‐triphosphate nucleotides, using commercially ...
Direct Attachment of Conjugate Groups to the 5′ Terminus of Oligodeoxyribonucleotides
(Wiley Periodicals, Inc., 2000-02)
This unit gives protocols for the attachment of intercalating and photoreactive conjugate groups to oligodeoxyribonucleotides. Protocols are given for acridine‐ and psoralen‐conjugated oligonucleotides, and include attachment ...
Determination of Binding Thermodynamics
(Wiley Periodicals, Inc.JAI Press, 2000-02)
This unit serves as a starting point for exploring the thermodynamic properties of interactions between small molecules and DNA. It covers the determination of simple, apparent association/dissociation constants. The ...
RNA Secondary Structure Prediction
(Kluwer Academic PublishersWiley Periodicals, Inc., 2000-10)
Details for predicting secondary structure of RNA sequences using free energy minimization are given. Protocols present the use of the computer program RNAstructure (for PCs) and the mfold server (for Unix platforms). The ...
DNA Synthesis Without Base Protection
(Wiley Periodicals, Inc.Columbia University Press, 2004-09)
DNA synthesis can be achieved by using O‐selective methods for internucleotide bond formation. This greatly simplifies the synthesis of oligodeoxyribonucleotides by eliminating the need for nucleobase protection and ...