Now showing items 101-110 of 160
Use of Fluorescence Spectroscopy to Elucidate RNA Folding Pathways
(Wiley Periodicals, Inc.W.H. Freeman and Company, 2002-06)
This overview unit discusses fluorescence spectroscopy as a tool for studying RNA folding. Ribozymes and oligonucleotides can be labeled with a fluorescent probe and analyzed to give information about both slow and fast ...
Probing RNA Structures with Hydroxyl Radicals
(Wiley Periodicals, Inc., 2000-02)
Fe(II)‐EDTA can be used to conveniently generate hydroxyl radicals to promote cleavage of RNA at nucleotide resolution. Two procedures are described, involving the generation of free radicals from solvated molecular oxygen ...
Thin‐Layer Chromatography
(Wiley Periodicals, Inc.CRC Press,, 2000-12)
TLC is used extensively in nucleic acid chemistry to monitor the progress of chemical reactions, to assay fractions collected from a larger chromatographic separation (e.g., column chromatography), and to determine optimal ...
Conjugation of 5′‐Functionalized Oligodeoxyribonucleotides with Properly Functionalized Ligands
(IRL PressWiley Periodicals, Inc., 2001-12)
This unit reports the synthesis of oligodeoxyribonucleotides covalently linked via their 5′ termini to various ligands such as intercalating agents, reactive groups, or labels. Methods for incorporation of halogenoalkyl, ...
Fluorinated α-aminophosphonates—a new type of irreversible inhibitors of serine hydrolases
(Nauka/Interperiodica; MAIK “Nauka/Interperiodica” ; Springer Science+Business Media, 2005-01)
Rapid Magnesium Chelation as a Method to Study Real‐Time Tertiary Unfolding of RNA
(Wiley Periodicals, Inc.MIT Press, 2001-09)
This unit describes a method to measure the unfolding of RNA tertiary structure on a millisecond time scale. A stopped‐flow spectrophotometer is used to measure the rate of unfolding induced by the addition of EDTA to an ...
Synthesis of 1,5‐Anhydrohexitol Building Blocks for Oligonucleotide Synthesis
(Wiley Periodicals, Inc., 2003-09)
This unit describes in detail, the optimized preparations of 1,5‐anhydrohexitol and the 1,5‐anhydrohexitol building blocks for oligonucleotide synthesis (hG, hA, hC, hT).
A Brief History, Status, and Perspective of Modified Oligonucleotides for Chemotherapeutic Applications
(Academic PressWiley Periodicals, Inc., 2000-02)
The advent of rapid and efficient methods of oligonucleotide synthesis has allowed the design of modified oligonucleotides that are complementary to specific nucleotide sequences in mRNA targets. Such modified oligonucleotides ...
Solid‐Phase Synthesis of 2′‐Deoxy‐2′‐fluoro‐ β‐D‐Oligoarabinonucleotides (2′F‐ANA) and Their Phosphorothioate Derivatives
(Wiley‐VCH, 2002-09)
This unit describes the chemical synthesis of 2’‐deoxy‐2’‐fluoro‐b‐D‐oligoarabinonucleotides (2’F‐ANA), both with phosphodiester and phosphorothioate linkages. The protocols described herein include araF phosphoramidite ...