Now showing items 131-140 of 160
Molecular Modeling of Nucleic Acid Structure: Energy and Sampling
(Wiley Periodicals, Inc.Oxford University Press, 2001-04)
An overview of computer simulation techniques as applied to nucleic acid systems is presented. This unit expands an accompanying overview unit (UNIT ) by discussing methods used to treat the energy and sample representative ...
Synthesis of Oligoribonucleotides Containing N6‐Alkyladenosine and 2‐Methylthio‐N6‐Alkyladenosine
(American Society for Microbiology PressWiley Periodicals, Inc., 2004-06)
The N6‐alkyladenosines and 2‐methylthio‐N6‐alkyladenosines are the most common modified adenosine nucleosides, and transfer ribonucleic acids (tRNA) are particularly rich in these modified nucleosides. They are present at ...
Probing RNA Structure and Function by Nucleotide Analog Interference Mapping
(Wiley Periodicals, Inc., 2004-06)
Nucleotide analog interference mapping (NAIM) can be used to simultaneously, yet individually, identify structurally or catalytically important functional groups within an RNA molecule. Phosphorothioate‐tagged nucleotides ...
Calorimetry of Nucleic Acids
(John Wiley & Sons, 2000-02)
This unit describes the application of differential scanning and isothermal titration calorimetry (DSC and ITC) to the study of the thermodynamics of nucleic acid structure. DSC is used to study order‐disorder transitions. ...
Synthesis of Protected 2′‐Deoxy‐2′‐fluoro‐β‐D‐arabinonucleosides
(Wiley Periodicals, Inc.International Society for Nucleosides, Nucleotides, and Nucleic Acids, 2002-09)
This unit describes in detail the preparation of protected 2’‐deoxy‐2’‐fluoroarabinonucleosides. These building blocks are required for the synthesis of 2’‐deoxy‐2’‐fluoroarabinonucleic acid (2’F‐ANA), an oligonucleotide ...
Probing RNA Folding Pathways by RNA Fingerprinting
(John Wiley & Sons, 2000-10)
This unit provides protocols for using native polyacrylamide gel electrophoresis to distinguish folding and unfolding conformers of RNA. It is useful for studying conformers that can exchange in a period of minutes or ...
Analyzing Radioligand Binding Data
(Raven Press,Wiley Periodicals, Inc., 2000-09)
A radioligand is a radioactively labeled drug that can associate with a receptor, transporter, enzyme, or any protein of interest. Measuring the rate and extent of binding provides information on the number of binding ...
Cartridge Methods for Oligonucleotide Purification
(Wiley Periodicals, Inc., 2000-07)
Protocols are given for purification of oligonucleotides by dimethoxytrityl‐sensitive and affinity desalting methods. The protocols are applicable for many of the convenient disposable products available for rapid ...
Chemistry of CpG DNA
(Wiley Periodicals, Inc., 2003-03)
The vertebrate immune system can recognize specific pathogen‐associated molecular patterns in invading microorganisms, including the unmethylated CpG dinucleotide. This unit discusses the receptors that recognize CpG motifs ...
Incorporation of Halogenoalkyl, 2‐Pyridyldithioalkyl, or Isothiocyanate Linkers into Ligands
(IRL PressWiley Periodicals, Inc., 2001-07)
Ligands can be introduced at the 5’ terminus of an oligonucleotide by adding a linker to the ligand and modifying the 5’ terminus of the oligonucleotide. These are then reacted to give the ligand‐oligonucleotide conjugate. ...