Now showing items 141-150 of 160
Raulin award lecture: Wilson's disease therapy with zinc and tetrathiomolybdate
(John Wiley & Sons, Inc., 2000)
Zinc and tetrathiomolybdate, effective, nontoxic drugs, have now been added to the treatment of most phases of Wilson's disease. Zinc is used for maintenance as well as treating presymptomatic, pregnant, and pediatric ...
Synthetic Strategies and Parameters Involved in the Synthesis of Oligodeoxyribo‐ and Oligoribonucleotides According to the H‐Phosphonate Method
(Wiley Periodicals, Inc.Humana Press, 2000-02)
Oligonucleotides can be synthesized by condensing a protected nucleoside H‐phosphonate monoester with a second nucleoside in the presence of a coupling agent to produce a dinucleoside H‐phosphonate diester. This can then ...
Analysis and Purification of Synthetic Nucleic Acids Using HPLC
(Wiley Periodicals, Inc.Stockton Press, 2000-07)
HPLC is a powerful and popular method for analyzing and purifying biomolecules. Reversed‐phase HPLC allows a high‐capacity method for purification, and uses volatile buffer systems that simplify product recovery. Anion‐exchange ...
A turn in the road: How studies on the pharmacology of glucosylceramide synthase inhibitors led to the identification of a lysosomal phospholipase A 2 with ceramide transacylase activity
(Kluwer Academic Publishers; Springer Science+Business Media, 2003-01)
A series of inhibitors of glucosylceramide synthesis, the PDMP based family of compounds, has been developed as a tool for the study of sphingolipid biochemistry and biology. During the course of developing more active ...
The 4â Methylthioâ 1â Butyl Group for Phosphate/Thiophosphate Protection in Oligodeoxyribonucleotide Synthesis
(Wiley Periodicals, Inc., 2004-12)
The detailed preparation of deoxyribonucleoside phosphoramidites functionalized with a 4â methylthioâ 1â butyl group for P(III) protection is described, along with the incorporation of these phosphoramidites into DNA ...
Commentary: Landmark articles on copper in the field of human health
(John Wiley & Sons, Inc., 2001)
No abstract.
Development of a Universal Nucleobase and Modified Nucleobases for Expanding the Genetic Code
(W.H. Freeman and Co.Wiley Periodicals, Inc., 2002-09)
This unit presents protocols for the synthesis and characterization of nucleosides with unnatural bases in order to develop bases for the expansion of the genetic alphabet or for nonselective pairing opposite natural bases. ...
Analysis of Oligonucleotides by Electrospray Ionization Mass Spectrometry
(Wiley Periodicals, Inc., 2000-02)
Because of the high molecular weights and thermal lability of biomolecules such as nucleic acids and protein, they can be difficult to analyze by mass spectrometry. Such analyses require a “soft” ionization method that is ...
Selection for Catalytic Function with Nucleic Acids
(Wiley Periodicals, Inc.Cold Spring Harbor, 2000-02)
For in vitro selection of catalytic polynucleotides, each new protocol must be designed to harness the desired catalytic activity to help propel the selection process itself. This unit gives guidelines for design of in ...
Attachment of Reporter and Conjugate Groups to the 3′ Termini of Oligonucleotides
(Wiley Periodicals, Inc.Humana Press, 2000-10)
Conjugation of oligonucleotides at the 3 terminus is less common because this site is used for covalent linkage to solid‐phase oligonucleotide synthesis supports. However, 3‐oligonucleotide conjugates have several valuable ...