Now showing items 151-160 of 160
Recent Advances in RNA Structure Determination by NMR
(John Wiley & Sons, 2000-10)
Despite recent advances in the solution of NMR structures of RNA and RNA‐ligand complexes, the rate limiting step remains the gathering of a large number of NOE and torsion restraints. Additional sources of information for ...
Analysis of Oxidized DNA Fragments by Gel Electrophoresis
(Wiley Periodicals, Inc., 2001-04)
Polyacrylamide gel electrophoresis is used to define and quantify products of deoxyribose oxidation in DNA, based on the unique electrophoretic mobility of DNA fragments possessing deoxyribose oxidation products on their ...
NMR Determination of Oligonucleotide Structure
(Oxford University PressWiley Periodicals, Inc., 2000-02)
This unit provides an overview of the use of NMR to determine oligonucleotide structure. It covers basic NMR spectral properties, acquisition of interproton distance restraints and torsion angle restraints, structure ...
Solid‐Phase Synthesis of Branched Oligonucleotides
(Wiley Periodicals, Inc.Academic Press, 2002-06)
Branched nucleic acids (bNAs) have been of particular interest since the discovery of RNA forks and lariats as intermediates of nuclear mRNA splicing, as well as multicopy, single‐stranded DNA (msDNA). Such molecules contain ...
Chemical and Enzymatic Methods for Preparing Circular Single‐Stranded DNAs
(CRC PressWiley Periodicals, Inc., 2000-02)
Small circular oligonucleotides can be used for diagnostic, therapeutic, and laboratory purposes. These systems have gained considerable attention in recent years because they form unusually strong and specific complexes ...
3′‐Modified Oligonucleotides and their Conjugates
(Wiley Periodicals, Inc.Pierce Chemical Company, 2000-12)
Solid‐phase synthesis of 3′‐aminoalkyl, 3′‐thioalkyl, and 3′‐polyethyleneglycol are described. The first two are important as specific points of attachment for a large variety of reporter groups; the latter is important ...
Probing Nucleic Acid Structure with Nickel‐ and Cobalt‐Based Reagents
(Kluwer Academic PublishersWiley Periodicals, Inc., 2000-02)
The use of nickel and cobalt reagents is presented for characterizing the solvent exposure of guanine residues in DNA and RNA. These reagents promote guanine oxidation in the presence of a peracid such as monopersulfate, ...
Engineering Disulfide Cross‐Links in RNA Via Air Oxidation
(Oxford University PressWiley Periodicals, Inc., 2000-02)
This unit presents protocols for the synthesis of alkylthiol‐modified ribonucleosides, their incorporation into synthetic RNA, and the formation of intramolecular disulfide bonds in RNA by air oxidation. The disulfide bonds ...
Introduction to the Synthesis and Purification of Oligonucleotides
(Applied BiosystemsWiley Periodicals, Inc., 2000-02)
Modern nucleic acid synthesizers utilize phosphite triester chemistries that employ stable phosphoramidite monomers to build a growing polymer. These robust reactions allow easy generation of specific oligodeoxyribo‐ and ...
Chemometric Methods for the Determination of Volatile Organic Compounds with Microsensor Arrays.
(2008)
This research addresses critical chemometric modeling and data analysis functions needed to guide the design and implementation of novel meso-scale and micro-scale instrumentation that incorporates chromatographic separation ...