Now showing items 11-20 of 160
Base Composition Analysis of Nucleosides Using HPLC
(Applied Biosystems,Wiley Periodicals, Inc., 2000-07)
In this protocol, nuclease digestion of an oligonucleotide is followed by dephosphorylation and HPLC analysis of the monomers on a reversed‐phase C18 column. This method can be used to detect and quantitate a wide variety ...
Chemoenzymatic Preparation of Nucleoside Triphosphates
(Wiley Periodicals, Inc., 2004-03)
The design and synthesis of alternative nucleoside triphosphate substrates for DNA and RNA polymerases holds continued promise to create biochemical probes and precursors for synthesis of nucleic acid mimics. The azole ...
Modification of the 5′ Terminus of Oligonucleotides for Attachment of Reporter and Conjugate Groups
(John Wiley & Sons, 2000-02)
Reporter and conjugate groups can be added directly to the 5′ terminus of oligonucleotides by appropriate modification. Conjugate groups can be used to increase the affinity of complementary strands, induce irreversible ...
Protection of 2′‐Hydroxy Functions of Ribonucleosides
(Oxford University PressWiley Periodicals, Inc., 2000-02)
The main purpose of this article is to discuss 2′‐protection in the context of effective oligoribonucleotide synthesis. Emphasis is placed on the 2′‐protecting groups of choice in the synthesis of oligo‐and polyribonucleotides, ...
Synthesis and Purification of Oligonucleotide N3′→︀P5′ Phosphoramidates and their Phosphodiester and Phosphorothioate Chimeras
(Wiley Periodicals, Inc., 2000-12)
This unit describes the synthesis and purification of oligonucleotide N3′→︀P5′ phosphoramidates, wherein each 3′‐oxygen is replaced by a 3′‐amine in the 2′‐deoxyribose ring. The synthesis of required monomers and application ...
Site‐Specific Fluorescent Labeling of Large RNAs with Pyrene
(Oxford University PressWiley Periodicals, Inc., 2004-12)
Pyrene is a useful chromophore for monitoring the tertiary structure and folding of large RNAs. This unit describes the general preparation of a large RNA (>80 nucleotides in length) that has been site‐specifically modified ...
Preparation of 2′‐O‐[(Triisopropylsilyl)oxy]methyl‐protected Ribonucleosides
(Oxford University PressWiley Periodicals, Inc., 2001-12)
The [(triisopropylsilyl)oxy]methyl (TOM) group is a useful protecting group for the 2′‐OH of ribonucleosides to be used for oligoribonucleotide synthesis by the phosphoramidite method. It is completely stable to all reaction ...
Synthesis of Phosphorothioate Oligonucleotides with Stereodefined Phosphorothioate Linkages
(Wiley‐Liss, 2003-09)
A method for solid‐phase synthesis of stereodefined PS‐oligos via an oxathiaphospholane approach using pure P‐diastereomers of nucleoside oxathiaphospholane monomers is described. The oxathiaphospholane monomers are ...