Now showing items 71-80 of 160
Synthesis of Endcap Dimethoxytrityl Phosphoramidites for Endcapped Oligonucleotides
(Wiley Periodicals, Inc., 2003-03)
Endcaps may be either aromatic or aliphatic molecules that specifically crossâ link the 5â ² end of one strand with the 3â ² end of the complementary strand in a DNA duplex. Endcaps may be viewed as a replacement of the ...
Selected Abbreviations Used in This Manual
(Wiley Periodicals, Inc., 2004-09)
This APPENDIX provides commonly used abbreviations for many terms used in in this manual.
Overview of the Synthesis of Nucleoside Phosphates and Polyphosphates
(Wiley‐Interscience, 2003-12)
This overview summarizes methodology used for the synthesis of nucleoside mono‐, di‐, and triphosphates. Selected techniques such as the Mitsunobu reaction, displacement reactions involving nucleoside 5′‐tosylates, ...
Reversible Biotinylation of the 5′‐Terminus of Oligodeoxyribonucleotides and its Application in Affinity Purification
(John Wiley & Sons, 2003-09)
The preparation of two reversible biotinylation phosphoramidites and their application in labeling and affinity purification of synthetic oligodeoxyribonucleotides will be described. In both cases, the biotin is linked to ...
Polyacrylamide Gel Electrophoresis (PAGE) of Synthetic Nucleic Acids
(Foster CityWiley Periodicals, Inc., 2000-07)
Protocols are given for analysis of oligonucleotides by PAGE, using either methylene blue staining or radiolabeling to mark the oligonucleotide. In addition, a separate protocol is provided for purification by PAGE.
2′‐Hydroxyl‐Protecting Groups that are Either Photochemically Labile or Sensitive to Fluoride Ions
(Wiley Periodicals, Inc., 2000-12)
Protected ribonucleotide monomers are more difficult to obtain than their 2′‐deoxy counterparts because of the need to protect the 2′‐hydroxy function. This unit describes the stepwise preparation of suitably 2′‐protected ...
Analysis of Oligonucleotides by Matrixâ Assisted Laser Desorption/Ionization Timeâ ofâ Flight Mass Spectrometry
(Wiley Periodicals, Inc., 2000-02)
MALDIâ MS is one of the most useful techniques available for determining biomolecule mass. It offers high mass accuracy, good sensitivity, simplicity, and speed. Because singly charged ions of oligonucleotides are typically ...
5′‐Iodination of Solid‐Phase‐Linked Oligodeoxyribonucleotides
(Wiley Periodicals, Inc., 2003-09)
5′‐Iodinated oligodeoxyribonucleotides readily react with 3′‐phosphorothioated DNA in the presence of a complementary template to yield a conjugate that is identical to natural DNA in every respect except that one oxygen ...
Probing RNA Structure and Metal‐Binding Sites Using Terbium(III) Footprinting
(John Wiley & Sons, 2003-06)
The function of an RNA molecule is determined by its overall secondary and tertiary structure. The tertiary structure is facilitated and stabilized by the interaction with metal ions. The current chapter offers a detailed ...
Evaluating hydrogen bond interactions in enzymes containing Mn(III)-histidine complexation using manganese-imidazole complexes
(Springer-Verlag; SBIC, 2003-02)
It is often difficult to control hydrogen bond interactions in small molecule compounds that model metalloenzyme active sites. The imidazole-containing ligands 4,5-dicarboxyimidazole (H 3 DCBI) and 4,5-dicarboxy- N ...