Now showing items 81-90 of 160
Deoxyribonucleoside Phosphoramidites
(Humana PressWiley Periodicals, Inc., 2001-04)
The detailed preparation of deoxyribonucleoside phosphoramidites bearing a 4‐[N‐methyl‐N‐(2,2,2‐trifluoroacetyl)amino]butyl group for P(III) protection is presented. The use of this group circumvents nucleobase alkylation ...
RNA Folding Pathways
(Wiley Periodicals, Inc., 2000-10)
A general overview of the questions and problems in RNA folding is presented. Topics include the differences in the folding problems/questions that apply to RNA versus proteins, methods for determination of final structures, ...
Probing RNA Structure by Lead Cleavage
(Halsted PressWiley Periodicals, Inc., 2000-02)
Lead cleavage causes a transesterification reaction that breaks the 5′,3′‐phosphodiester backbone of RNA, leaving a 2′,3′‐cyclic phosphate and a 5′‐hydroxyl. Since the efficiency of the reaction at the 2′‐hydroxyl is related ...
Strategies for Oligoribonucleotide Synthesis According to the Phosphoramidite Method
(John Wiley & Sons, 2000-02)
Advances in oligoribonucleotide synthesis have lagged behind those in oligodeoxyribonucleotide synthesis because of the difficulty in identifying orthogonal protecting groups for the 2′‐ and 5′‐hydroxyls. Adaptation of the ...
Capillary Electrophoresis of DNA
(John Wiley & Sons, 2003-06)
Capillary electrophoresis (CE) is an alternative to conventional slab gel electrophoresis for the separation of DNA fragments. CE offers a number of advantages over slab gel separations in terms of speed, resolution, ...
Chemical and Enzymatic Probes for Nucleic Acid Structure
(Wiley Periodicals, Inc., 2001-12)
Purification and Analysis of Synthetic Nucleic Acids and Components
(Wiley Periodicals, Inc., 2001-04)
Preparation of α‐Oxo Semicarbazone Oligonucleotide Microarrays
(Wiley Periodicals, Inc.Oxford University Press, 2004-12)
This unit describes the preparation of α‐oxo aldehyde functionalized oligodeoxynucleotides, the preparation and characterization of semicarbazide glass slides, and the fabrication of α‐oxo semicarbazone microarrays by ...
Attachment of Nucleosides to Solid‐Phase Supports
(ESCOMWiley Periodicals, Inc., 2000-02)
Specific step‐by‐step instructions are given for coupling nucleosides to LCAA‐CPG supports (supports consisting of a long‐chain alkylamine linked to controlled‐pore glass). Protocols are given for a succinic acid linker ...