Now showing items 101-110 of 410
Probing DNA Structure with Hydroxyl Radicals
(Wiley Periodicals, Inc., 2001-12)
The hydroxyl radical is a useful probe for studying the shape of the surface of a DNA molecule. Using this technique, fine details of DNA structure can potentially be revealed. This unit describes how to use the hydroxyl ...
Engineering Terminal Disulfide Bonds Into DNA
(Wiley Periodicals, Inc.Springer‐Verlag, 2003-06)
This protocol presents a simple and general means of modifying nucleic acids with disulfide cross‐links. These cross‐links serve as powerful tools to probe the structure, dynamics, thermodynamics, folding, and function of ...
Design, Synthesis, and Amplification of DNA Pools for In Vitro Selection
(PerSeptive BiosystemsWiley Periodicals, Inc., 2000-02)
Preparation of a random‐sequence DNA pool is presented. The degree of randomization and the length of the random sequence are discussed, as is synthesis of the pool using a DNA synthesizer. Purification of a single‐stranded ...
Synthesis of Oligoribonucleotides Using the 2‐Nitrobenzyloxymethyl Group for 2′‐Hydroxyl Protection
(Wiley Periodicals, Inc., 2000-12)
RNA can be rapidly synthesized using protected ribonucleoside phosphoramidite monomers that carry 2‐nitrobenzyloxymethyl groups on their 2‐hydroxyls. These protecting groups are removed from the oligoribonucleotide products ...
Statistical mechanics of protein folding with separable energy functions
(Wiley Subscription Services, Inc., A Wiley Company, 2004-06-15)
We have initiated an entirely new approach to statistical mechanical models of strongly interacting systems where the configurational parameters and the potential energy function are both constructed so that the canonical ...
Chemistry of Minor Groove Binder–Oligonucleotide Conjugates
(CRC PressWiley Periodicals, Inc., 2003-06)
Various types of minor groove binders have been attached to synthetic oligodeoxynucleotides, and the interactions of these conjugates (MB‐ODNs) with DNA are reviewed here. MB‐ODNs have enhanced DNA affinity and have improved ...
Model-based synthesis of nonlinear PI and PID controllers
(Wiley Subscription Services, Inc., A Wiley Company, 2001-08)
PI and PID controllers continue to be popular methods in industrial applications. It is well known that linear PI and PID controllers result from the application of model-based controller design methods to linear first- ...
Water-density effects on phenol oxidation in supercritical water
(Wiley Subscription Services, Inc., A Wiley Company, 2003-03)
Supercritical water oxidation (SCWO) experiments were performed in a tubular flow reactor at 420–465°C and 141–241 bar. Phenol was the organic reactant. Both pure water and a helium (1/3 by mol)–water mixture served as ...
Backbone dynamics determined by electron paramagnetic resonance to optimize solid-phase peptide synthesis of TOAC-labeled phospholamban
(Wiley Subscription Services, Inc., A Wiley Company, 2007)
Electron paramagnetic resonance (EPR) was used to optimize the solid-phase peptide synthesis of a membrane-bound peptide labeled with TOAC (2,2,6,6-tetramethyl-piperidine-1-oxyl-4-amino-4-carboxylic acid). The incorporation ...
Large volume, high-performance applications of fibers in civil engineering
(Wiley Periodicals, Inc., 2002-01-18)
This article presents an overview of fiber applications in cementitious composites. The socio-economic considerations surrounding materials development in civil engineering in general, and fiber reinforced cementitious ...