Now showing items 41-50 of 121
A novel method of high‐purity extracellular vesicle enrichment from microliter‐scale human serum for proteomic analysis
(Endotext, MDText.com, Inc.Wiley Periodicals, Inc., 2021-02)
We have developed a rapid, low‐cost, and simple separation strategy to separate extracellular vesicles (EVs) from a small amount of serum (i.e.,<100 μL) with minimal contamination by serum proteins and lipoprotein particles ...
The pH- Induced Selectivity Between Cysteine or Histidine Coordinated Heme in an Artificial α- Helical Metalloprotein
(SpringerWiley Periodicals, Inc., 2021-02-19)
De Novo metalloprotein design assesses the relationship between metal active site architecture and catalytic reactivity. Herein, we use an α- helical scaffold to control the iron coordination geometry when a heme cofactor ...
Making or Breaking Metal- Dependent Catalytic Activity: The Role of Stammers in Designed Three- Stranded Coiled Coils
(Wiley Periodicals, Inc.ACS Publications, 2020-11-09)
While many life- critical reactions would be infeasibly slow without metal cofactors, a detailed understanding of how protein structure can influence catalytic activity remains elusive. Using de novo designed three- ...
Hydrazone and Oxime Olefination via Ruthenium Alkylidenes
(Wiley, 2022-05-23)
We describe the development of an efficient method for the olefination of hydrazones and oximes. The key design approach that enables this transformation is tuning of the energy/polarity of C=N π-bonds by employing heteroatom ...
Synergetic effect of aramid nanofiber‐graphene oxide hybrid filler on the properties of rubber compounds for tire tread application
(John Wiley & Sons, Inc., 2022-04-05)
The properties of rubber compounds used in tire tread largely contribute to the overall performance of tires in vehicles. Among the various ingredients used, reinforcing fillers are known for having the most significant ...
Layered Biomimetic Composites from MXenes with Sequential Bridging
(Wiley Periodicals, Inc., 2022-03-21)
Layer‐by‐layer (LbL) assembly is a widely used technique for the self‐assembly of layered nanocomposites from clay sheets, carbon nanotubes, graphene oxide, nanoparticles, and other materials. Other approaches for fabricating ...
Direct comparison of Eulerian–Eulerian and Eulerian–Lagrangian simulations for particle‐laden vertical channel flow
(John Wiley & Sons, Inc., 2020-07)
Particle‐laden flows in a vertical channel were simulated using an Eulerian–Eulerian, Anisotropic Gaussian (EE‐AG) model. Two sets of cases varying the overall mass loading were done using particle sizes corresponding to ...
FRACPRED- 2D- PRM: A Fraction Prediction Algorithm- Assisted 2D Liquid Chromatography- Based Parallel Reaction Monitoring- Mass Spectrometry Approach for Measuring Low- Abundance Proteins in Human Plasma
(Wiley Periodicals, Inc., 2020-12)
Multidimensional fractionation- based enrichment methods improve the sensitivity of proteomic analysis for low- abundance proteins. However, a major limitation of conventional multidimensional proteomics is the extensive ...
Rapid microsphere‐assisted peptide screening (MAPS) of promiscuous MHCII‐binding peptides in Zika virus envelope protein
(John Wiley & Sons, Inc., 2020-03)
Despite promising developments in computational tools, peptide‐class II MHC (MHCII) binding predictors continue to lag behind their peptide‐class I MHC counterparts. Consequently, peptide–MHCII binding is often evaluated ...
Control of Stereoselectivity in Diverse Hapalindole Metabolites is Mediated by Cofactor‐Induced Combinatorial Pairing of Stig Cyclases
(Wiley Periodicals, Inc.Academic Press, 2020-05-18)
Stereospecific polycyclic core formation of hapalindoles and fischerindoles is controlled by Stig cyclases through a three‐step cascade involving Cope rearrangement, 6‐exo‐trig cyclization, and a final electrophilic aromatic ...